Screening of Varicella-Zoster virus specific CTL antigen and analysis of Varicella vaccine induced cell-mediated immunity.

• Project/Area Number: 24790456
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Kinki University
• Principal Investigator: KANAI Kyosuke 近畿大学, 医学部, 助教 (20596621)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 水痘帯状疱疹ウイルス(VZV) / 感染防御 / 細胞性免疫 / 水痘帯状疱疹ウイルス

Research Abstract

Cell-mediated immunity (CMI) is thought to be crucial for protection against Varicella-Zoster virus (VZV) infection. We screened for major CMI-inducible VZV protein by Enzyme-Linked Immuno-Spot (ELISPOT) assay. Because already-known CMI antigens of other herpesviruses are localized in cell nuclei, nuclear distributed VZV proteins were examined as candidates for major CMI antigens. VZV protein(s) inducing CMI were searched among peripheral blood mononuclear cell specimens derived from healthy volunteers. As the result, ORF8, 61 and 66 induced CMI in more than 80% of specimens. About ORF61, epitope sequences were identified using oligo peptides. Our findings contribute to establish a new standardized CMI-quantification system.

Research Products

• [Journal Article] Presence of Epstein-Barr virus-infected B lymphocytes with thyrotropin receptor antibodies on their surface in Graves' disease patients and in healthy individuals (2014)
  • Author(s): Nagata K, Higaki K, Nakayama Y, Miyauchi H, Kiritani Y, Kanai K, Matsushita M, Iwasaki T, Sugihara H, Kuwamoto S, Kato M, Murakami I, Nanba E, Kimura H, Hayashi K
  • Journal Title: Autoimmunity Volume: 47(3) Pages: 193-200
  • Peer Reviewed
• [Journal Article] Presence of Epstein-Barr virus-infected B lymphocytes with thyrotropin receptor antibodies on their surface in Graves' disease patients and in healthy individuals. (2014)
  • Author(s): Nagata K, Higaki K, Nakayama Y, Miyauchi H, Kiritani Y, Kanai K, Matsushita M, Iwasaki T, Sugihara H, Kuwamoto S, Kato M, Murakami I, Nanba E, Kimura H, Hayashi K.
  • Journal Title: Autoimmunity. Volume: 47(3) Issue: 3 Pages: 193-200
  • DOI: 10.3109/08916934.2013.879863
  • Peer Reviewed
• [Journal Article] EBNA-2 -deleted Epstein-Barr virus from P3HR-1 can infect rabbits with lower efficiency than prototype Epstein-Barr virus from B95-8. (2013)
  • Author(s): Sano H, Nagata K, Kato K, Kanai K, Yamamoto K, Okuno K, Kuwamoto S, Higaki-Mori H, Sugihara H,Kato M, Murakami I, Kanzaki S, Hayashi K.
  • Journal Title: Intervirology Volume: 56 Issue: 2 Pages: 114-21
  • DOI: 10.1159/000343753
  • Peer Reviewed
• [Presentation] 水痘帯状疱疹ウイルス特異的CTL抗原の探索 (2012)
  • Author(s): 金井亨輔,谷口留美,湯華民,森康子,井上直樹
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ(愛知県)(14)
  • Year and Date: 2012-06-08
• [Presentation] 水痘帯状疱疹ウイルス特異的細胞性免疫を誘導する抗原の探索 (2012)
  • Author(s): 金井亨輔,谷口留美,湯華民,森康子,井上直樹
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)(P-061)
• [Presentation] 水痘帯状疱疹ウイルス特異的細胞性免疫を誘導する抗原の探索 (2012)
  • Author(s): 金井亨輔,湯華民,森康子,井上直樹
  • Organizer: 第60回日本ウイルス学科九学術集会
  • Place of Presentation: グランキューブ大阪(大阪府)(O1-B-08)
• [Presentation] O1-B-08 水痘帯状疱疹ウイルス特異的細胞性免疫を誘導する抗原の探索 (2012)
  • Author(s): 金井亨輔、湯華民、森康子、井上直樹
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪 (大阪府)
• [Presentation] P-061 水痘帯状疱疹ウイルス特異的細胞性免疫を誘導する抗原の探索 (2012)
  • Author(s): 金井亨輔、谷口留美、湯華民、森康子、井上直樹
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜 (神奈川県)
• [Presentation] 14 水痘帯状疱疹ウイルス特異的CTL抗原の探索
  • Author(s): 金井亨輔、谷口留美、福井良子、湯華民、森康子、井上直樹
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ (愛知県)