Endothalial cell-derived IL-7 controls B cell development and peripheral T cell maintenance

• Project/Area Number: 24790468
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: HARA Takahiro 京都大学, ウイルス研究所, 助教 (90512301)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: サイトカイン / IL-7 / 内皮細胞 / T細胞 / Ｂ細胞 / B細胞

Research Abstract

IL-7 is an essential cytokine for the development, proliferation and survival of lymphocytes. However, the precise distribution and the local function of IL-7-expressing cells are largely unknown. In this research project, I aimed to clarify the effects of IL-7 produced by lymphatic endothelial cells and bone marrow endothelial cells on peripheral T cells and bone marrow B cells. By using endothelial cell specific IL-7 deleted mice, I found that endothelial cell-derived IL-7 controls the peripheral T cell migration and bone marrow B cell development.

Research Products

• [Journal Article] Characterization of the interleukin-15 niche in primary and secondary lymphoid organs in vivo. (2014)
  • Author(s): Cui G, Hara T, Simmons S, Wagatsuma K, Abe A, Miyachi H, Kitano S, Ishii M, Tani-ichi S, Ikuta K.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 5 Pages: 1915-1920
  • DOI: 10.1073/pnas.1318281111
  • Peer Reviewed
• [Journal Article] Interleukin-7 produced by thymic epithelial cells plays a major role in the development of thymocytes and TCRγδ+ intraepithelial lymphocytes. (2013)
  • Author(s): Shitara S, Hara T, Liang B, Wagatsuma K, Zuklys S, Hollander GA, Nakase H, Chiba T, Tani-ichi S, Ikuta K.
  • Journal Title: J Immunol Volume: 190 Issue: 12 Pages: 6173-6179
  • DOI: 10.4049/jimmunol.1202573
  • Peer Reviewed
• [Journal Article] B cell progenitors and precursors change their microenvironment in fetal liver during early development. (2013)
  • Author(s): Tsuneto M, Tokoyoda K, Kajikhina E, Hauser AE, Hara T, Tani-ichi S, Ikuta K, Melchers F.
  • Journal Title: Stem Cells Volume: 31 Issue: 12 Pages: 2800-2812
  • DOI: 10.1002/stem.1421
  • Peer Reviewed
• [Journal Article] CD49b-dependent estabhshment of T helper cell memory. (2013)
  • Author(s): Hanazawa, A., Hayashizaki, K.,Shinoda, K., Yagita, H., Okumura, K., Löhning, M., Hara, T., Tani-ichi, S., Ikuta, K., Eckes, B.,Radbruch, A., Tokoyoda, K, and Nakayama, T.
  • Journal Title: Immuno1. Cell Bio1 Volume: 91 Issue: 8 Pages: 524-531
  • DOI: 10.1038/icb.2013.36
  • Peer Reviewed
• [Journal Article] Interleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations. (2013)
  • Author(s): Tani-Ichi, S., Shimba, A., Wagatsuma, K., Miyachi, H., Kitano, S., Imai, K., Hara, T., and Ikuta, K.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Issue: 2 Pages: 612-617
  • DOI: 10.1073/pnas.1219242110
  • NAID: 120005241695
  • Peer Reviewed
• [Journal Article] IL-7産生細胞のリンパ組織内分布 (2013)
  • Author(s): 原崇裕、生田宏一
  • Journal Title: 医学のあゆみ Volume: Vol.246、No.12
• [Journal Article] Identification of IL-7-producing cells in primary and secondary lymphoid organs using IL-7-GFP knock-in mice. (2012)
  • Author(s): Hara, T., S. Shitara, K. Imai, H. Miyachi, S. Kitano, H. Yao, S. Tani-Ichi, and K. Ikuta
  • Journal Title: Journal of Immunology Volume: 189 Issue: 4 Pages: 1577-1584
  • DOI: 10.4049/jimmunol.1200586
  • Peer Reviewed
• [Journal Article] Role of hepatocyte-derived IL-7 in maintenance of intrahepatic NKT cells and T cells and development of B cells in fetal liver. (2012)
  • Author(s): Liang, B., T. Hara, K. Wagatsuma, J. Zhang, K. Maki, H. Miyachi, S. Kitano, C. Yabe-Nishimura, S. Tani-Ichi, and K. Ikuta
  • Journal Title: Journal of Immunology Volume: 189 Issue: 9 Pages: 4444-4450
  • DOI: 10.4049/jimmunol.1201181
  • Peer Reviewed
• [Presentation] Distribution and function of IL-7-expressing cells in intestines (2013)
  • Author(s): Takahiro Hara, Guangwei Cui, Soichiro Shitara, and Koichi Ikuta
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2013-12-11
• [Presentation] Visualization and characterization of IL-7- and IL-15-expressing cells in vivo (2013)
  • Author(s): Takahiro Hara, Guangwei Cui, and Koichi Ikuta
  • Organizer: International Workshop of Kyoto T Cell Conference 2013
  • Place of Presentation: 京都大学芝蘭会館
• [Presentation] Visualization and characterization of IL-7- and IL-15-expressing cells in vivo (2013)
  • Author(s): Hara Takahiro, Cui Guangwei, Ikuta Koichi
  • Organizer: International Workshop of Kyoto T Cell Conference 2013
  • Place of Presentation: 京都大学 芝蘭会館
• [Presentation] Visualization and Characterization of Homeostatic Cytokine Expressing Cells In Vivo (2013)
  • Author(s): Hara Takahiro, Cui Guangwei, Ikuta Koichi
  • Organizer: 6th International Workshop of Kyoto T Cell Conference
  • Place of Presentation: 京都大学 芝蘭会館
• [Presentation] DISTRIBUTION AND FUNCTION OF IL-7-EXPRESSING CELLS IN THE INTESTINAL TISSUES (2012)
  • Author(s): Takahiro Hara, Koichi Ikuta
  • Organizer: The Third Workshop of Synthetic Immunology
  • Place of Presentation: 京都大学 芝蘭会館
• [Presentation] Distribution and function of IL-7-expressing cells in intestines
  • Author(s): HARA Takahiro, CUI Guangwei, SHITARA Soichiro, IKUTA Koichi
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ
• [Presentation] DISTRIBUTION AND FUNCTION OF IL-7-EXPRESSING CELLS IN THE INTESTINAL TISSUES
  • Author(s): Takahiro Hara, Koichi Ikuta
  • Organizer: 第8回研究所ネットワーク国際シンポジウム
  • Place of Presentation: 京都大学 芝蘭会館
• [Presentation] Distribution and function of IL-7-expressing cells in large intestine
  • Author(s): Takahiro Hara, Koichi Ikuta
  • Organizer: The Fourth Workshop of Synthetic Immunology
  • Place of Presentation: 京都大学 楽友会館
• [Book] 感染・炎症・免疫 第４３巻 (2014)
  • Author(s): 生田宏一、原崇裕、谷一靖江
  • Total Pages: 3
  • Publisher: 鳥居薬品株式会社
• [Book] 医学のあゆみ Vol.246 (2013)
  • Author(s): 原崇裕、生田宏一
  • Total Pages: 2
  • Publisher: 医歯薬出版株式会社
• [Remarks]
  • URL: http://www.virus.kyoto-u.ac.jp/Lab/ikuta.html
• [Remarks] 京都大学ウイルス研究所
  • URL: http://www.virus.kyoto-u.ac.jp/
• [Remarks] 京都大学ウイルス研究所 生体防御研究分野
  • URL: http://www.virus.kyoto-u.ac.jp/virus/seitaibougyo.html
• [Remarks] 京都大学ウイルス研究所
  • URL: http://www.virus.kyoto-u.ac.jp/
• [Remarks] 京都大学ウイルス研究所 生体防御研究分野
  • URL: http://www.virus.kyoto-u.ac.jp/virus/seitaibougyo.html