Identification of functional relationship between the ILCs and the mast cells in the small intestine

• Project/Area Number: 24790474
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: LEE YOUNGAE 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (60610681)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: IL-22 / IL-17F / DEFAs / Innate lymphoid cells / Mast cells / Small intestine / α-defensins / Mast cell progenitors / 自然リンパ系細胞（ILC / マスト細胞前駆体 / 小腸 / マスト細胞プロテアーゼ / 細胞分化 / マスト細胞

Research Abstract

We found that the IL-7Ra+ population (CD4- LTi-like cells) within Lin-c-Kit+NKp46-CD4- cells has a protective role against bacterial infection through high expression of IL-17F and IL-22. The strong expression of DEFAs by the IL-7Ra-CD34-b7int population in the steady-state condition may play an important role in maintaining tissue homeostasis with commensal bacteria. IL-7Ra-CD34+b7int and IL-7Ra-CD34intb7hi cells are MCPs, which can differentiate into MCs that may regulate inflammatory responses by expressing high levels of MCPT5 and CPA3.

Research Products

• [Journal Article] Role of Mouse and Human Autophagy Proteins in IFN-γ-Induced Cell-Autonomous Responses against Thxoplasma gondii (2014)
  • Author(s): Jun Ohshima, Youngae Lee, Miwa Sasai, Tatsuya Saitoh, Ji Su Ma, Naganori Kamiyama, Yoshiharu Matsuura, Suh Pann-Ghill, Mikako Hayashi, Shigeyuki Ebisu, Kiyoshi Takeda, Shizuo Akira, and Masahiro Yamamoto.
  • Journal Title: The Journal of Immunology Volume: 192(7) Issue: 7 Pages: 3328-35
  • DOI: 10.4049/jimmunol.1302822
  • Peer Reviewed / Open Access
• [Journal Article] Intestinal Lin-c-Kit+NKp46-CD4- Population Strongly Produces IL-22 upon IL-1β Stimulation (2013)
  • Author(s): Youngae Lee, Yutaro Kumagai, Min Seong Jang, Jung-Hwan Kim, Bo-Gie Yang, Eun-Jung Lee, You-Me Kim, Shizuo Akira, and Myoung Ho Jang
  • Journal Title: The Journal of Immunology Volume: 190 Issue: 10 Pages: 5296-5305
  • DOI: 10.4049/jimmunol.1201452
  • Peer Reviewed
• [Journal Article] Journal of Dermatological Science (2012)
  • Author(s): Hee Jin Byun, Kwang Hyun Cho, Hee Chul Eun, Min-Jung Lee, Youngae Lee, Serah Lee, Jin Ho Chung
  • Journal Title: Lipid ingredients in moisturizers can modulate skin responses to UV in barrier-disrupted human skin in vivo. Volume: 65 Issue: 2 Pages: 110-117
  • DOI: 10.1016/j.jdermsci.2011.12.005
  • Peer Reviewed
• [Presentation] Intestinal Lin^-c-Kit^+NKp46^-CD4^ - population highly produces IL-22 upon IL-1βstimulation (2013)
  • Author(s): Y Lee, Y Kumagai, MS Jang, JH Kim, BG Yang, S Akira, and MH Jang
  • Organizer: The American Association of Immunologists (AAI)
  • Place of Presentation: Hawaii, USA
• [Presentation] Intestinal Lin-c-Kit+NKp46-CD4- population highly produces IL-22 upon IL-1β stimulation (2013)
  • Author(s): Youngae Lee, Yutaro Kumagai, Shizuo Akira, and Myoung Ho Jang
  • Organizer: AAI
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Intestinal mast cell progenitors function as innate lymphoid cells (2012)
  • Author(s): Y Lee, Y Kumagai, S Akira, and MH Jang
  • Organizer: The American Association of Immunologists (AAI)
  • Place of Presentation: Boston, USA
• [Presentation] Intestinal mast cell progenitors function as innate lymphoid cells. (2012)
  • Author(s): Youngae Lee
  • Organizer: Immunology 2012, AAI Annual meeting
  • Place of Presentation: Hynes Convention Center (Boston, Massachusetts, USA)