| Project/Area Number |
24790475
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
TAKADA Kensuke 徳島大学, 疾患プロテオゲノム研究センター, 講師 (40570073)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 獲得免疫 / T細胞 / 正の選択 / 胸腺 / 免疫学 |
|---|
| Research Abstract |
beta5t-containing thymoproteasomes expressed in cortical thymic epithelial cells (cTECs) are essential for positive selection of CD8 T cells. In this study, we characterized monoclonal CD8 T cells that were selected in the absence of beta5t. We found that OT-I CD8 T cells generated in beta5t-deficient mice were hyperresponsive to SIINFEKL antigen and its variants. In the periphery, CD8 T cells generated in beta5t-deficient mice were enriched with CD44hiCD122hi memory-phenotype depending on IL-2/15 receptor signals, suggesting that beta5t-dependent positive selection is required for homeostatic maintenance of CD8 T cells. In response to infection with Listeria monocytogenes expressing ovalbumin, OT-I CD8 T cells from beta5t-deficient mice preferentially differentiated into short-lived effector cells. These results indicate that the positive selection in the thymus not only determines TCR recognition specificity but also preconditions antigen responsiveness of CD8 T cells.
|
