Clarifying the mechanism of B cell tolerance by CIN85-mediated BCR regulation

Research Project

Project/Area Number	24790487
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Immunology
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	KOMETANI Kohei 独立行政法人理化学研究所, 統合生命医科学研究センター, 研究員 (50437258)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	免疫寛容 / B細胞 / CIN85
Research Abstract	To verify the possibility that adaptor molecule CIN85, which is required for transducing B Cell Receptor (BCR) signalling, have a role in the induction and maintenance of immune tolerance in B cells, I established a below experimental system. First, I generated B cell specific CIN85-deficient mice using mb1-Cre knock in mice and flox-CIN85 mice. Second, CIN85 B cell specific deficient mice were crossed with mice those have a BCR recognising Hen Egg Lysozyme (HEL) and the mice expressing soluble HEL antigen.

Report

(3 results)