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Synthesis of [18F]labelled proteins using cell-free system

Research Project

Project/Area Number 24790527
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied pharmacology
Research InstitutionTohoku University

Principal Investigator

SHIBUYA Katsuhiko  東北大学, 医学(系)研究科(研究院), 教育研究支援者 (30596268)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsイメージング / 無細胞蛋白質合成 / ポジトロン断層撮影 / IL-8 / 18F
Research Abstract

First, we synthesized [19F]-interleukin-8 (IL-8) which contained non-labelled fluoroproline instead of proline using the cell-free protein synthesis system. We confirmed that pure and abundant [19F]-IL-8 could be obtained. The binding affinity of [19F]IlL-8 to IL8 receptors was retained. Next, we synthesized [18F]IL-8 using [18F]proline. After ion-exchanged chromatography, we could obtained [18F]IL-8 which had 91% of radiochemical purity in a short time.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Mechanism of the histamine H3 receptor-mediated increase in exploratory locomotor activity and anxiety-like behaviours in mice2014

    • Author(s)
      Mohsen A, Yoshikawa T, Miura Y, Nakamura T, Naganuma F, Shibuya K, Iida T, Harada R, Okamura N, Watanabe T, Yanai K.
    • Journal Title

      Neuropharmacology

      Volume: 81 Pages: 188-94

    • DOI

      10.1016/j.neuropharm.2014.02.003

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] [ (11)C] Doxepin binding to histamine H1 receptors in living human brain: reproducibility during attentive waking and circadian rhythm2012

    • Author(s)
      KatsuhikoShibuya
    • Journal Title

      FrontiersinSystemsNeuroscience

      Volume: 6 Pages: 1-7

    • DOI

      10.3389/fnsys.2012.00045

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 頚部痛および肩こり患者における用手療法施術後の脳および骨格筋糖代謝変化 : [18F]FDG-PET研究2012

    • Author(s)
      稲見暁惠、小倉毅、田代学、四月朔日聖一、マスド・メヘディ、渋谷勝彦、三宅正泰、平岡宏太良、伊藤正敏、山口慶一郎、谷内一彦
    • Organizer
      第52回日本核医学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-10-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 頚部痛および肩こり患者における用手療法施術後の脳および骨格筋糖代謝変化:[18F]FDG-PET研究2012

    • Author(s)
      稲見暁惠、小倉毅、田代学、四月朔日聖一、マスド・メヘディ、渋谷勝彦、三宅正泰、平岡宏太良、伊藤正敏、山口慶一郎、谷内一彦
    • Organizer
      第52回日本核医学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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