| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In drug delivery system (DDS), the modification of nanocarriers with polyethylene glycol (PEGylated nanocarrier) is commonly used technique to improve blood circulation time. However, it is reported that repeated injection of PEGylated carrier cause ‘accelerated blood clearance (ABC)’ phenomenon in animal experiments. The ABC phenomenon is one of the barriers in the DDS development. In this study, the convenient evaluation method using in vivo confocal microscopy was established. After the investigation of various nanocarrier, the structure avoiding the ABC phenomenon was examined. The evaluation method of siRNA intact ratio including the nanocarrier and the inhibition method of photobleaching in case of long time measurement were also established.
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