Basic investigation of detection targeted at resistant-factors of drug-resistant microorganisms
Project/Area Number |
24790551
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
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Research Institution | Nagoya University |
Principal Investigator |
KEIKO Yamada 名古屋大学, 医学(系)研究科(研究院), 助教 (00402561)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 薬剤耐性 / 検出 / 抗体 / 感染症 / 検査 / 迅速 / MRSA |
Outline of Final Research Achievements |
In this study, we aimed to developed specific antibodies against resistant-factor for rapid detection of drug-resistant microorganisms. In this study, target molecules are key proteins of drug-resistance, 1) PBP2a of methicillin resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MRCNS), 2) IMP-1 of carbapenem-resistant bacteria, and 3) oseltamivir- and peramivir-resistant type neuraminidase of influenza virus. I have prepared specific antibodies for them, and they showed the specificity with recombinant proteins and resistant/susceptible microorganisms. I constructed immunochromatography assay system using specific anti-PBP2a chicken IgY. It was revealed that it could detected MRSA and MRCNS directly from mimicked blood-cultured bottle in about 3 hours.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Molecular epidemiological characteristics of Klebsiella pneumoniae associated with bacteremia among patients with pneumonia.2015
Author(s)
R. Ito, Y. Shindo, D. Kobayashi, M. Ando, W. Jin, J. Wachino, K. Yamada, K. Kimura, T. Yagi, Y. Hasegawa, Y. Arakawa
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Journal Title
J Clin Microbiol.
Volume: 53
Issue: 3
Pages: 879-889
DOI
Related Report
Peer Reviewed
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[Journal Article] Detection of Methicillin-Resistant <i>Staphylococcus aureus</i> Using a Specific Anti-PBP2a Chicken IgY Antibody2013
Author(s)
Yamada K, Wanchun J, Ohkura T, Murai A, Hayakawa R, Kinoshita K, Mizutani M, Okamoto A, Namikawa T, Ohta M.
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Journal Title
Japanese Journal of Infectious Diseases
Volume: 66
Issue: 2
Pages: 103-108
DOI
NAID
ISSN
1344-6304, 1884-2836
Related Report
Peer Reviewed
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[Journal Article] Complete genome sequence of Bacillus cereus NC7401, which produces high levels of the emetic toxin cereulide.2012
Author(s)
Takeno A, Okamoto A, Tori K, Oshima K, Hirakawa H, Toh H, Agata N, Yamada K, Ogasawara N, Hayashi T, Shimizu T, Kuhara S, Hattori M, Ohta M
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Journal Title
J Bacteriol
Volume: 194
Issue: 17
Pages: 4767-4768
DOI
Related Report
Peer Reviewed
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