| Project/Area Number |
24790553
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
SATO Hiaki 岡山大学, 保健学研究科, 助教 (70362960)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 成人T細胞白血病/リンパ腫（ATLL） / SHP1 / 成人T細胞白血病/リンパ腫（ATL） / 癌性幹細胞 / Epigenetics / 異常DNAメチル化 / HTLV－1 |
|---|
| Outline of Final Research Achievements |
We analyzed to clarify the multistep tumorigenic events in adult T cell leukemia/lymphoma (ATLL) by focusing on SHP1. SHP1 is a nonreceptor type protein-tyrosine phosphatase, which acts as a negative regulator in hematopoietic cells. In immunostaining, the expression of SHP1 and tumor-related genes decreased in various ATLL-related cell lines. By performing methylation specific PCR (MSP) assay, we detected aberrant methylation of SHP1 and these genes. Moreover, we identified specific aberrant DNA methylation profile in ATLL-related cell lines. Our findings demonstrate that the presence of aberrant methylation is deeply involved in the development and progression of ATLL in multistep leukemogenesis.
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