| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we analyzed the seroreactivities of patients with cat scratch disease (CSD) and of healthy subjects against the VirB5 and BepA subtypes of Bartonella henselae. We developed an ELISA for measurement of anti-VirB5 antibody levels and a serotyping method for the VirB5 subtype. The sensitivity and specificity of the ELISA relative to an indirect fluorescence antibody (IFA) assay were 72.6% and 99.0%, respectively. For patients with positive results for anti-VirB5 antibodies, the serotyping method could identify the VirB5 subtype as being the strain causing infection. The VirB5 ELISA and the serotyping method for the VirB5 subtype are expected to be useful tools for molecular epidemiological studies of B. henselae infection.
|
