Comprehensive study for detection of coronary endothelial dysfunction using microRNA array analysis

• Project/Area Number: 24790559
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Nagoya City University
• Principal Investigator: Sugiura Tomonori 名古屋市立大学, 医学(系)研究科(研究院), 助教 (60535235)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 動脈硬化 / マイクロRNA / 血管内皮 / 酸化ストレス / 高血圧 / 喫煙 / 脂質異常 / 冠動脈 / 狭心症 / 内皮障害 / microRNA / セロトニン / micorRNA / 冠動脈疾患 / 探索的研究

Outline of Final Research Achievements

Vascular endothelial damage was improved and plasma levels of circulating microRNA-126 were increased after 8 weeks of smoking cessation, demonstrating variable circulating levels of microRNA in different conditions. The ratio of plasma/whole blood serotonin concentration showed significant association with oxidative stress in individuals with cardiovascular risks, and is a significant determinant of the incidence of coronary artery disease. Targeting serotonin-oxidative stress axis as a part of a holistic anti-atherothrombotic strategy is potentially benefitial in patients with atherosclerosis. Moreover, damages in the coronary endothelium affect circulating levels of endothelium-derived microRNAs. Further examination is necessary to establish the clinical significance of microRNAs.

Research Products

• [Journal Article] Serotonin in Peripheral Blood Reflects Oxidative Stress and Plays a Crucial Role in Atherosclerosis: Novel Insights toward Holistic Anti-atherothrombotic Strategy. (2016)
  • Author(s): Sugiura T, Yamashita S, Hirowatari Y, Fujii S, Ohte N.
  • Journal Title: Atherosclerosis Volume: 246 Pages: 157-160
  • DOI: 10.1016/j.atherosclerosis.2016.01.015
  • Peer Reviewed
• [Journal Article] Circulating Level of MicroRNA-126 may be a Potential Biomarker for Recovery from Smoking-Related Vascular Damage in Middle-Aged Habitual Smokers. (2015)
  • Author(s): Tomonori Sugiura, Yasuaki Dohi, Sumiyo Yamashita, Soichiro Iwaki, Shiori Ito, Akimasa Sanagawa, Nobuyuki Ohte, Satoshi Fujii
  • Journal Title: IJC Heart & Vasculature Volume: 印刷中 Pages: 83-87
  • DOI: 10.1016/j.ijcha.2015.02.012
  • Peer Reviewed / Open Access
• [Presentation] Plasma Serotonin Reflects Oxidative Stress and Plays a Crucial Role in Atherosclerosis: Novel Insights Toward Holistic Anti-atherothrombotic Strategy (2016)
  • Author(s): 杉浦知範
  • Organizer: 第80回日本循環器学会総会・学術集会
  • Place of Presentation: 仙台国際会議場
  • Year and Date: 2016-03-18
• [Presentation] Plasma Serotonin Reflects Oxidative Stress and Plays a Crucial Role in Atherosclerosis: Novel Insights toward Holistic Anti-atherothrombotic Strategy. (2015)
  • Author(s): Tomonori Sugiura
  • Organizer: Scientific Session of American Heart Association 2015
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research