The establishment of 'novel clinical examination for rheumatoid arthritis' by focusing on acetylation of proteins
Project/Area Number |
24790563
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
ARITO Mitsumi 聖マリアンナ医科大学, 医学部, 助教 (00509911)
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Co-Investigator(Renkei-kenkyūsha) |
NAGAI Kouhei 近畿大学, 生物理工学部, 講師 (70500578)
SATO Toshiyuki 聖マリアンナ医科大学, 医学部, 助教 (10350430)
KUROKAWA Manae 聖マリアンナ医科大学, 医学部, 准教授 (90301598)
OKAMOTO Kazuki 聖マリアンナ医科大学, 医学部, 准教授 (40177085)
KATO Tomohiro 聖マリアンナ医科大学, 医学部, 教授 (80233807)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | プロテオミクス / 翻訳後修飾 / アセチル化 / 関節リウマチ / 臨床検査 |
Research Abstract |
Post-translational modifications (PTMs) are often critical for diagnosis of diseases. Thereby, we here tried to elucidate alteration of PTMs in RA, focusing on acetylation in this study. We applied acetyl-proteomics to peripheral blood mononuclear cells (PBMCs) to elucidate PTM difference between patients with RA and healthy. Multiple proteins in PBMCs were highly acetylated in the RA groups. One of the proteins predominantly acetylated in the RA group was identified to be ENO1. Next, we tried to identified acetylated lysine residues, but we could not detect RA-specific acetylated peptides of ENO1. This would be technical limitation of mass spectrometric analysis at present. In the future, it would be needed to establish more effective methods to identify acetylated lysine residues. Next, we examined whether acetylation affect the antigenecity of ENO1 in patients with RA. As a result, the reactivity was not different between the acetylated ENO1 and the non-acetylated ENO1.
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Report
(3 results)
Research Products
(69 results)
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[Journal Article] Proteomic analysis of whole glomeruli in patients with IgA nephropathy using microsieving.2014
Author(s)
Kojima S, Koitabashi K, Iizuka N, Okamoto K, Arito M, Sato T, Kurokawa MS, Suematsu N, Shibagaki Y, Yasuda T, Kimura K, Kato T
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Journal Title
American journal of nephrology.
Volume: 39
Issue: 1
Pages: 36-45
DOI
Related Report
Peer Reviewed
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[Journal Article] Roles of serum fibrinogen α chain-derived peptides in Alzheimer's disease.2013
Author(s)
Noguchi M, Sato T, Nagai K, Utagawa I, Suzuki I, Arito M, Iizuka N, Suematsu N, Okamoto K, Kato T, Yamaguchi N, Kurokawa MS.
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Journal Title
International Journal of Geriatric Psychiatry
Volume: in press
Issue: 8
Pages: 808-818
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Novel anti-citrullinated peptide autoantibodies identified by proteomics with in vitro citrullinated proteins in patients with rheumatoid arthritis2013
Author(s)
Mitsui H, Arito M, Sato T, Yokoyama MK, Suematsu N, Okamoto K, Kurokawa MS, Yudo K, Nakamura H, Beppu M, Kato T
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Journal Title
Inflammation and Regeneration
Volume: 33
Pages: 121-130
NAID
Related Report
Peer Reviewed
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[Journal Article] Protein profiles of peripheral blood mononuclear cells as a candidate biomarker for Behcet’s disease.2013
Author(s)
Yoshioka T, Kurokawa MS, Sato T, Nagai K, Iizuka N, Arito M, Takakuwa Y, Nakano H, Ooka S, Suematsu N, Okamoto K, Yudoh K, Nakamura H, Suzuki N, Ozaki S, Kato T
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Journal Title
Clin Exp Rheumatol
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Effect of sulfasalazine and tofacitinib on the protein profile of articular chondrocytes.2013
Author(s)
Endo W, Arito M, Sato T, Kurokawa MS, Omoteyama K, Iizuka N,Okamoto K, Suematsu N, Nakamura H, Beppu M, Kato T.
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Journal Title
Modern Rheum.
Volume: in press
Issue: 5
Pages: 844-850
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Serum peptides, represented by complement 3f des-arginine, are useful for prediction of the response to pegylated interferon-α plus ribavirin in patients with chronic hepatitis C2013
Author(s)
Yohei Noguchi, Manae S. Kurokawa, Chiaki Okuse, Nobuyuki Matsumoto, Kouhei Nagai, Toshiyuki Sato, Mitsumi Arito, Naoya Suematsu, Kazuki Okamoto, Michihiro Suzuki, Fumio Itoh, Tomohiro Kato
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Comprehensive analysis of aberrantly glycosylated proteins in rheumatoid arthritis2013
Author(s)
Sato T, Arito M, Kurokawa MS, Takakuwa Y, Ooka S, Nagai K, Nakamura H, Iizuka N, Suematsu N, Okamoto K, Kato T.
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Serum peptide profiles as a candidate biomarker for dementia with Lewy bodies2013
Author(s)
Itsuku Suzuki, Manae S. Kurokawa, Miwa Noguchi, Toshiyuki Sato, Itaru Utagawa, Mitsumi Arito, Nobuko Iizuka, Kazuki Omoteyama, Naoya Suematsu, Kazuki Okamoto, Noboru Yamaguchi, Tomohiro Kato
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Protein profiles of peripheral blood mononuclear cells as a biomarker for Behcet’s disease2013
Author(s)
Takuya Yoshioka, Kurokawa MS, Sato T, Nagai K, Iizuka N, Arito M, Takakuwa Y, Nakano H, Ooka H, Suematsu N, Okamoto K, Nakamura H, Suzuki N, Ozaki S, Kato T.
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Comparative croteomic analysis of neutrophils from patients with microscopic polyangiitis and granulomatosis with polyangiitis.2013
Author(s)
Uchida T, Nagai K, Sato T, Iizuka N, Arito M, Takakuwa Y, Nakano H, Ooka S, Kurokawa MS, Suematsu N, Okamoto K, Ozaki S, Kato T.
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Proteomic analysis of whole glomeruli in patients with IgA nephropathy using micro-sieving2013
Author(s)
Kojima S, Koitabashi K, Iizuka N, Okamoto K, Arito M, Sato T, Kurokawa MS, Suematsu N, Yasuda T, Kimura K, Kato T
Organizer
HUPO2013
Place of Presentation
神奈川県横浜市(パシフィコ横浜)
Related Report
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[Presentation] Protein profiles of peripheral blood mononuclear cells as a biomarker for Behcet’s Disease.2013
Author(s)
Kurokawa MS, Yoshioka T, Sato T, Nagai K, Iiduka N, Arito M, Takakuwa Y, Nakano H, Ooka S, Suematsu N, Okamoto K, Nakamura H, Suzuki N, Ozaki S, Kato T.
Organizer
American College of Rheumatology. Annual meeting 13
Place of Presentation
San Diego)
Related Report
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