Elucidation and application of the 5FU resistant mechanism of breast cancer using proteomic analysis

• Project/Area Number: 24790565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Osaka Medical College
• Principal Investigator: KIMURA Kosei 大阪医科大学, 医学部, 助教 (20623846)
• Research Collaborator: SAKAI Akiko 大阪医科大学, 医学部, 講師 ; TANAKA Satoru 大阪医科大学, 医学部, 助教 ; FUJIOKA Hiroya 大阪医科大学, 医療総合研修センター臨床研修室, 臨床研修専任指導医
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 化学療法 / 薬物耐性 / 乳癌 / 抗癌剤耐性 / プロテオーム解析 / 悪性腫瘍 / 薬剤感受性

Outline of Final Research Achievements

We performed proteomic analysis to 5FU resistant colon cancer cell line and identified some proteins (HP27, Prohibitin, TFAM) as candidate associated 5FU resistance. We showed a positive association between HP27 and disease progression by an evaluation using breast cancer serum sample. We abandoned an analysis for Prohibitin and TFAM due to loss of their reproducibility. We also identified some candidate proteins for Taxane resistance by proteomic analysis using Taxane resistant breast cancer cell line.