| Project/Area Number |
24790568
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 臨床化学 / 抗体工学 / ハプテン / 抗メタタイプ抗体 / scFv / バイオマーカー |
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| Research Abstract |
There are various small molecules (haptens) in blood or urine which are useful as biomarker for clinical diagnosis. However, conventional immunoassays are not always applicable for sensitive determination of such haptens due to insufficient affinity of anti-hapten antibodies. Anti-metatype antibodies, which bind specifically to antigen-antibody complex, have been applied for sandwich-type noncompetitive immunoassays. However, the traditionally protocol of immunization cannot easily generate the anti-metatype antibodies. In this study, we have selected scFvs (single-chain Fv fragments) as acceptor molecules for a target hapten to generate suitable complexes to induce anti-metatype antibodies. We have also investigated utility of the other acceptor: for example, sdAbs (single-domain antibodies) and aptamers.
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