| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
Diarrhea was induced by stress loaded with communication box in BALB/c mouse, although diarrhea was not caused in C57BL/6J mouse. Irritable bowel syndrome produces some symptoms similar to those of inflammatory bowel disease. In the present study, the gene expression of inflammatory cytokines in colon of the stress-loaded BALB/c mouse was examined. The expressions of various cytokines were attenuated by stress-loaded, although IL-4 and IL-17a gene expressions were increased by acute stress loaded, respectively. However, augmentation of these gene expressions was not correlated with development of diarrhea. On the other hand, gene expressions of TRPA1 and TRPV1 were increased by acute stress loaded. Furthermore, gene expression of cystic fibrosis transmembrane conductance regulator (CFTR) was increased by stress loaded. These results showed that the mechanism of stress-induced diarrhea is complicated in inflammation. Further studies are needed about involvement of TRPA1, TRPV1 and CFTR.
|
