The role of O-GlcNAc modification in aging-related deterioration of Toll-like receptor signaling in macrophages
Project/Area Number |
24790593
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hygiene
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Research Institution | Waseda University |
Principal Investigator |
SHIRATO Ken 早稲田大学, 人間科学学術院, 助手 (60559384)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 老化 / 加齢 / マクロファージ / リポ多糖 / 炎症 / Toll様受容体 / シグナル伝達 / 翻訳 / ヘキソサミン生合成経路 / 糖鎖修飾 |
Research Abstract |
In the present study, to clarify the molecular mechanism of age-related deterioration of macrophage inflammatory responses, lipopolysaccharide (LPS) responsiveness of peritoneal macrophages were compared between 2-month-old and 12-month-old mice. In middle age, the production of pro-inflammatory cytokine in response to LPS was reduced at the protein level but not at the mRNA level. In addition, Toll-like receptor signaling was not affected in middle age. In contrast, the phosphorylation level of eukaryotic initiation factor (eIF)-2alpha was increased in middle age. These results suggest that macrophage inflammatory responses were deteriorated at the post-transcriptional level in middle age, and that the age-related phenomena are associated with the reduction of mRNA translation mediated by increased phosphorylation of eIF-2alpha.
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Report
(3 results)
Research Products
(55 results)
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[Journal Article] Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise2013
Author(s)
Sato, S., Shirato, K., Mitsuhashi, R., Inoue, D., Kizaki, T., Ohno, H., Tachiyashiki, K. & Imaizumi, K
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Journal Title
J. Physiol. Sci
Volume: 63
Issue: 3
Pages: 211-218
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of exercise on HIF-1 and VEGF signaling2012
Author(s)
Ohno, H., Shirato, K., Sakurai, T., Ogasawara, J., Sumitani, Y., Sato, S., Imaizumi, K., Ishida, H. and Kizaki, T
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Journal Title
The Journal of Physical Fitness and Sports Medicine
Volume: 1
Issue: 1
Pages: 5-16
DOI
NAID
ISSN
2186-8123, 2186-8131
Related Report
Peer Reviewed
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[Journal Article] Effects of β<sub>2</sub>-agonists and exercise on β<sub>2</sub>-adrenergic receptor signaling in skeletal muscles2012
Author(s)
Sato, S., Shirato, K., Kizaki, T., Ohno, H., Tachiyashiki, K. and Imaizumi, K
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Journal Title
The Journal of Physical Fitness and Sports Medicine
Volume: 1
Issue: 1
Pages: 139-144
DOI
NAID
ISSN
2186-8123, 2186-8131
Related Report
Peer Reviewed
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[Journal Article] Age-related effects of fasting on ketone body productions during lipolysis in rats2012
Author(s)
Higashino-Matsui Y, Shirato K, Suzuki Y, Kawashima Y, Someya Y, Sato S, Shiraishi A, Jinde M, Matsumoto A, Ideno H, Tachiyashiki K, Imaizumi K
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Journal Title
Environmental Health and Preventive Medicine
Volume: 17
Issue: 2
Pages: 157-163
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Sensitivity of Heterozygous α1,6-Fucosyltransferase Knock-out Mice to Cigarette Smoke-induced Emphysema: IMPLICATION OF ABERRANT TRANSFORMING GROWTH FACTOR-β; SIGNALING AND MATRIX METALLOPROTEINASE GENE EXPRESSION.2012
Author(s)
Gao C, Maeno T, Ota F, Ueno M, Korekane H, Takamatsu S, Shirato K, Matsumoto A, Kobayashi S, Yoshida K, Kitazume S, Ohtsubo K, Betsuyaku T, Taniguchi N
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Journal Title
J. Biol. Chem.
Volume: 287(20)
Issue: 20
Pages: 16699-16708
DOI
Related Report
Peer Reviewed
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