Analysis of the effects of oxidative stress on DNA methylation and the expression of active DNA demethylation related genes
| Project/Area Number |
24790600
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
TAKUMI Shota 東京慈恵会医科大学, 医学部, 助教 (80570770)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 酸化ストレス / ＤＮＡ脱メチル化 / エピジェネティクス / ＤＮＡメチル化 / DNAメチル化 / DNA脱メチル化 / パラコート |
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| Research Abstract |
In this study, we investigated the effects of oxidative stress on DNA methylation and the expression of active DNA demethylation related genes. The results of this study showed that paraquat-induced oxidative stress increased the expression of Aid, which is DNA demethylation related gene. Furthermore, over-expression of Aid increased some genes expression. Taken together, these results may suggest that the oxidative stress-induced activation of Aid is involved in those genes activation through the DNA demethylation. Further analysis of promoter methylation of Aid-activated genes will help to understand the effect of oxidative stress on DNA methylation.
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Report
(3 results)
Research Products
(13 results)
