| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Research Abstract |
Current methods, based on the detection of the Y-chromosome, can directly identify an unknown sample as male, but female gender is determined indirectly, by not detecting the Y-chromosome. Therefore, we developed a novel sex determination method by analyzing the number of X-chromosomes using a copy number variation (CNV) detection technique (the comparative Ct method). In this study, we designed a primer set using the amelogenin-X gene without the CNV region as the target to determine the X-chromosome copy number. All DNA samples from participants (20 males, 20 females) were evaluated correctly using this method with 1-ng template DNA. A minimum of 0.2-ng template DNA was found to be necessary for accurate sex determination with this method. Thus, we successfully developed a method of sex determination based on the number of X-chromosomes. Our novel method will be useful in forensic practice for sex determination.
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