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Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region

Research Project

Project/Area Number 24790650
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Legal medicine
Research InstitutionNational Research Institute of Police Science

Principal Investigator

WATANABE Ken  科学警察研究所, 法科学第一部, 研究員 (20532047)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords物体検査 / 人体液斑の同定 / DNAメチル化
Research Abstract

In this study, we analyzed aged stains of various body fluids by bisulfite sequence analysis for the methylation status in the previously reported semen-specific hypomethylated region, DACT1. The results indiate that the methylation status is stable in each old stain and that this can be a useful marker for forensic semen identification. We further developed a TaqMan realtime PCR detection method to make it easier to analyze the methylation status of DACT1 region. This method can be a useful supplementary tool for semen identification, especially from aged stains.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2013 Other

All Presentation (2 results)

  • [Presentation] DNA脱メチル化部位を指標とした精液証明法に関する予備的検討2013

    • Author(s)
      渡邊賢,阿久津智子,櫻田宏一
    • Organizer
      第82回日本法医学会学術関東地方集会
    • Place of Presentation
      神奈川
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA脱メチル化部位を指標とした精液証明法に関する予備的検討

    • Author(s)
      渡邊賢、阿久津智子、櫻田宏一
    • Organizer
      第82回日本法医学会学術関東地方集会
    • Place of Presentation
      横浜市西区みなとみらい
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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