| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
3,5-dichlorotyrosine was synthesized, and we developed an analytical method to
detect 3-chlorotyrosine and 3,5-dichlorotyrosine using LC-MS. The mothod uses a general reversed-phase column or a mix-mode column of normal-phase and cation exchange. When we apply the method to normal human urine, chlorinated tyrosines were under detection limit. Although we also apply the method to pooled normal human plasma after hydrolyzing plasma protein with methansulfonic acid, chlorinated tyrosines were also under detection limit. On the other hand, chlorinated tyrosines were detected in the normal human plasma after exposure to chlorine gas. It was also observed that the amount of generated chlorinated tyrosines depends on concentration of chlorine gas exposed to the human plasma.
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