Effect of fetal membrane-derived mesenchymal stem cells in rats with acute pancreatitis

• Project/Area Number: 24790670
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: KUWATANI Masaki 北海道大学, 大学病院, 助教 (50431375)
• Research Collaborator: KAWAKUBO Kazumichi 北海道大学, 大学院医学研究科, 助教 (80633578) ; OHNISHI Shunsuke 北海道大学, 北海道大学病院, 講師 (10443475)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 間葉系幹細胞 / 膵炎 / 卵膜 / 急性膵炎モデル

Outline of Final Research Achievements

Objectives: We investigated the effect of rat FM-derived mesenchymal stem cells (FM-MSCs) and human amnion-derived MSCs (AMSCs) on the therapeutic effects in rats with acute and chronic pancreatitis.
Methods: Acute pancreatitis was induced by intraductal injection of taurocholate, and rat FM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and human AMSCs were transplanted intravenously.
Results: Transplantation of rat FM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. Human AMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.