The elucidation about the relevance between the function of autophagy in the intestinal tract tissues and the intestinal tract inflammation pathology

• Project/Area Number: 24790671
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Asahikawa Medical College
• Principal Investigator: INABA Yuhei 旭川医科大学, 医学部, その他 (30447099)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オートファジー / 腸管上皮 / 炎症性腸疾患 / 腸管炎症 / サイトカイン / 実験腸炎モデル

Research Abstract

The expression of autophagy on cell line level and mouse level was used for the purpose of the elucidation of the relevance between the function of autophagy in intestinal tract tissues and the pathology of intestinal tract inflammation. In cell line level, the expression of autophagy was checked by induction of inflammatory cytokines. Examination is under continuation about the time course and dose dependency which the expression of autophagy increase by several cytokines. I plan to study about the expression of autophagy by induction of various anti-inflammatory substances. In the mouse level, I checked that the expression of autophagy was increased significantly in the DSS colitis models. And the expression of autophagy decreased in IL-10 knockout mice compared with wild type mice. I created the autophagy knockout mouse which had used for purpose of functional analysis.

Research Products

• [Journal Article] microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1. (2014)
  • Author(s): Fujiya M, Konishi H, Kamel M.K.M, Ueno N, Inaba Y, Moriichi K, Tanabe H, Ikuta K, Ohtake T, Kohgo Y.
  • Journal Title: Oncogene Volume: in press Issue: 40 Pages: 4847-56
  • DOI: 10.1038/onc.2013.429
  • Peer Reviewed
• [Journal Article] microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1 (2013)
  • Author(s): Fujiya M, Konishi H, Kamel M.K.M, Ueno N, Inaba Y, Moriichi K, Tanabe H, Ikuta K, Ohtake T, Kohgo Y
  • Journal Title: Oncogene
  • Peer Reviewed
• [Journal Article] Reduction of E-cadherin by Human Defensin-5 in Esophageal Squamous Cells (2013)
  • Author(s): Nomura Y, Tanabe H, Moriichi K, Igawa S, Ando K, Ueno N, Kashima S, Tominaga M, Goto T, Inaba Y, Ito T, Ishida-Yamamoto A, Fujiya M, Kohgo Y
  • Journal Title: Biochem Bioph Res Co
  • Peer Reviewed
• [Journal Article] Infliximab extends the duration until the first surgery in patients with Crohn’s disease. (2013)
  • Author(s): Sakatani A, Fujiya M, Ito T, Inaba Y, Ueno N, Kashima S, Tominaga M, Moriichi K, Okamoto K, Tanabe H, Ikuta K, Ohtake T, Kono T, Furukawa H, Ashida T, Kohgo Y.
  • Journal Title: Biomed Research International Volume: 2013 Pages: 879491-879491
  • DOI: 10.1155/2013/879491
  • NAID: 120006517749
  • Peer Reviewed
• [Journal Article] Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection (2012)
  • Author(s): Okamoto K, Fujiya M, Nata T, Ueno N, Inaba Y, Ishikawa C, Ito T, Moriichi K, Tanabe H, Mizukami Y, Chang EB, Kohgo Y
  • Journal Title: Int J Colorectal Dis
  • Peer Reviewed
• [Presentation] The novel probiotic function through activation of intestinal epithelial autophagy (2013)
  • Author(s): Inaba Y, Fujiya M, Kohgo Y
  • Organizer: 16th International Congress of Mucosal Immunology
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2013-07-17
• [Presentation] 腸管オートファジー誘導による抗酸化作用、腸管バリア増強作用を応用した過敏性腸症候群の新規治療開発 (2012)
  • Author(s): 稲場勇平、藤谷幹浩、高後裕
  • Organizer: 第54回日本消化器病学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-10-12
• [Presentation] Activation of intestinal epithelial autophagy as a potential and novel mechanism of probiotic action in the gut (2012)
  • Author(s): Inaba Y, Fujiya M, Kohgo Y
  • Organizer: ECCO2012
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2012-02-17
• [Presentation] The novel probiotic function through activation of intestinal epithelial autophagy
  • Author(s): Inaba Y, Fujiya M, Kohgo Y.
  • Organizer: 16th International Congress of Mucosal Immunology (ICMI 2013)
  • Place of Presentation: カナダ
• [Presentation] 当科で経験した潰瘍性大腸炎に 合併したcolitic cancerの検討
  • Author(s): 稲場勇平、藤谷幹浩、田中一之、堂腰達矢、坂谷 慧、安藤勝祥、上野伸展、伊藤貴博、盛一健太郎、高後 裕．
  • Organizer: 第54回日本消化器病学会大会
  • Place of Presentation: 東京