Analysis of a role for RASAL1 in colon carcinogenesis

• Project/Area Number: 24790681
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: ISOMURA Yoshihiro 東京大学, 医学部附属病院, 臨床登録医 (10599004)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: RASAL1 / 大腸がん / RASGAP / 遺伝子改変マウス

Outline of Final Research Achievements

RASAL1 knockout mice has been successfully established in this project. RASAL1 homo deficient mice were not embryonic lethal and did not develop any tumors in intestine, stomach or liver by 18 month after birth. RASAL1 hetero deficient mice were crossed to APC hetero deficient mice that spontaneously develop intestinal tumors, and the phenotypic effects in coloncarcinogenesis are under investigation. Also RASAL1 hetero deficient mice were treated with AOM/DSS and the effects in inflammation- triggered carcinogenesis are to be analyzed.