| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of this study is to identify tumor-suppressive microRNAs (miRNA) of pancreatic cancer by functional screening assay, a phenotype-oriented screening method, and their functional analysis, which may lead to a new strategy of the treatment of pancreatic cancer. Microarray was designed to detect each clone contained in the virus library which expresses microRNA precursors. MicroRNAs that suppress proliferation of pancreatic cancer were identified by semi-quantitative comparison of each clone before and after introduction of miRNA library either in vitro or in vivo. Among these miRNAs was microRNA-34 (miR-34): one of the mechanisms of the tumor-suppressive action appears to be through cell cycle pathway, which was shown by gene expression analysis of pancreatic cell line transfected with miR-34.
|
