Molecular mechanism of GIST related to malignant behavior

• Project/Area Number: 24790689
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: FUNASAKA Kohei 名古屋大学, 医学部附属病院, 病院助教 (70599034)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: GIST / 悪性化 / マイクロアレイ / KIT / ETV1 / ANKRD36BP2

Outline of Final Research Achievements

We conducted a research to explore new genes involved in malignant behavior in GIST. Firstly, mutated or wild type c-kit gene was transfected exogenously to 293T cell line. Extracted mRNA was subjected to microarray analysis in order to compare with the influence of each kit gene. More than 1000 genes had significantly changed by exogenous c-kit. 11 genes were dramatically up or down regulated more than ten times. Their expressions were evaluated in actual GIST sample. However, there were no genes related to malignancy. From this result, we next analyzed clinical GIST samples which are classified to benign or malignancy by using a microarray. Then nine genes had significantly increased their expressions in malignant group. New candidate genes which are related to malignant features will be found by analyzing in actual many kinds of GIST samples.

Research Products

• [Presentation] GISTに対するEUS-FNAを用いた遺伝子解析 (2015)
  • Author(s): 舩坂好平、宮原良二、後藤秀実
  • Organizer: 日本消化器内視鏡学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-05-29
• [Presentation] 当院におけるGIST の自然経過の検討 (2015)
  • Author(s): 舩坂好平、宮原良二、後藤秀実
  • Organizer: 日本消化管学会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-13
• [Presentation] Novel Biomarker Candidates for predicting high-risk GIST (2014)
  • Author(s): Kohei Funasaka, Ryoji Miyahara, Kazuhiro Furukawa, Takeshi Yamamura, Eizaburo Ohno, Masanao Nakamura, Hiroki Kawashima, Yoshiki Hirooka, Osamu Watanabe, Osamu Maeda, Takafumi Ando, Hidemi Goto
  • Organizer: APDW2014
  • Place of Presentation: バリ、インドネシア
  • Year and Date: 2014-11-25
• [Presentation] GISTにおける新規遺伝子マーカーの探索 (2014)
  • Author(s): 舩坂好平、宮原良二、古川和宏、山本富美子、松崎一平、横山敬史、菊池正和、浅井裕充、山村健史、大野栄三郎、中村正直、川嶋啓揮、廣岡芳樹、渡辺修、前田修、安藤貴文、後藤秀実
  • Organizer: JDDW2014
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2014-10-23
• [Presentation] Molecular Characteristics of Malignant GIST (2014)
  • Author(s): Kohei Funasaka, Ryoji Miyahara, Kazuhiro Furukawa, Eizaburo Ohno, Masanao Nakamura, Hiroki Kawashima, Akihiro Itoh, Yoshiki Hirooka, Osamu Watanabe, Osamu Maeda, Takafumi Ando, Hidemi Goto
  • Organizer: DDW2014
  • Place of Presentation: シカゴ、アメリカ
  • Year and Date: 2014-05-03
• [Presentation] GIST遺伝子解析と臨床的悪性度の比較検討 (2013)
  • Author(s): 舩坂好平
  • Organizer: 日本消化器病学会総会
  • Place of Presentation: 城山観光ホテル（鹿児島市）
• [Presentation] 転写因子ETV1によるPDGFRA発現とMAPK活性化の新しい知見 (2012)
  • Author(s): 舩坂好平
  • Organizer: 日本消化器関連学会週間2012（JDDW2012)
  • Place of Presentation: 神戸国際展示場（神戸市）
• [Presentation] c-kit expression could be a predictor of high risk GIST.
  • Author(s): Funasaka K, Miyahara R, Furukawa K, Ohno E, Nakamura M, Kawashima H, Itoh A, Hirooka Y, Watanabe O, Maeda O, Ando T, Goto H
  • Organizer: Taiwan Digestive Disease Week 2013
  • Place of Presentation: 台北 台湾
• [Presentation] EUS-FNAを用いたGIST遺伝子解析の有用性と問題点
  • Author(s): 舩坂好平、宮原良二、古川和宏、鶴留一誠、山本富美子、松崎一平、横山敬史、菊池正和、大野栄三郎、中村正直、川嶋啓揮、伊藤彰浩、大宮直木、廣岡芳樹、渡辺修、前田修、安藤貴文、後藤秀実
  • Organizer: JDDW2013 第55回日本消化器病学会大会
  • Place of Presentation: 新高輪グランドホテル 東京都港区