| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We have created the inflammation carcinogenesis model. As a method, 6-8-week-old male mice were injected with Azoxymethane (10 mg / kg) in an intraperitoneal manner, followed by a single cycle of Dextran Sulfate Sodium Salt (2.0%, 7 days) in drinking water and euthanized at 18 weeks. The study was done to evaluate by measuring the number and size of the tumor in colon. Mice were dissected, and we observed large intestine by stereomicroscope and histology. Using this model, we were able to study apoptosis, regeneration and growth mechanism of epithelial cells in inflammatory carcinogenesis processes. However, we did not lead to elucidate Lgr5 positive cells whether to act as a cancer stem cell in inflammatory carcinogenesis process. We think requires further study.
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