Establishment of 3D organotipic culture to identify the gastric stem cells, and to apply for the elucidation of mechanism of gastric cancer and for the novel therapy to gastric cancer
| Project/Area Number |
24790715
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胃癌 / 幹細胞 / オルガノイド / Helicobacter / 3次元培養 / 発癌 / 抗癌剤感受性 / 予後予測 |
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| Research Abstract |
In order to analyze the novel mechanism of gastric carcinogenesis, and to invent the novel therapy to gastric cancer, we performed the following experiments; 1) to establish the 3D-organotipic culture by using gastric epithelial cell, 2) to analyze the gene expression profile, and 3) to verify the tumorigenic property of gastric organoid.
As a results, we could establish the gastric organoid from primary gastric cells, and isolated many novel genes associated with intestinal metaplasia/carcinogenesis. We ould also clarify the tumorigenic property of organoid, which would contribute to gastric cancer.
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Report
(3 results)
Research Products
(3 results)
