Analysis of mechanism of maintaining the stemness properties in human colon cancer stem cells using innovative spheroid culture system

• Project/Area Number: 24790727
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: OHATA Hirokazu 独立行政法人国立がん研究センター, 研究所, 研究員 (40570057)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん幹細胞 / CD44 / 大腸がん

Research Abstract

It has been proposed that cancer stem cells (CSCs) are responsible for tumor formation, therapeutic resistance and recurrence. We established the in vitro spheroid culture of CSCs from human colon cancer surgical specimens. Therefore, through our analysis of the mechanism of maintaining the stemness properties in human colon CSCs, we aimed to link our study to the development of innovative cancer therapies.

Research Products

• [Journal Article] Induction of the stem-like cell regulator CD44 by Rho kinase inhibiition con ntributes to the mmaintenance of colon-initiating cells (2012)
  • Author(s): Hirokazu Ohata, Tatsuyya Ishiguro, Yuki Aihhara, Ai Sato, Hiroaki Sakai, Shgeki Sekine, Hirokkazu Taniguchi. Takayuki Akasu, Shin Fujita, Hitoshi Nakagama, , Koji Okaamoto
  • Journal Title: Cancer Research Volume: vol.72 Pages: 5101-55110
  • Peer Reviewed
• [Journal Article] Induction of the stem-like cell regulator CD44 by Rho kinase inhibition contributes to the maintenance of colon cancer-initiating cells. (2012)
  • Author(s): Hirokazu Ohata
  • Journal Title: Cancer Research Volume: 72 Issue: 19 Pages: 5101-5110
  • DOI: 10.1158/0008-5472.can-11-3812
  • Peer Reviewed
• [Presentation] 大腸がん幹細胞における Akt-mTOR経路を介したCD44の発現制御機構 (2013)
  • Author(s): 大畑広和、中釜斉、岡本康司
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 大腸がん幹細胞におけるAkt-mTOR経路を介したCD44の発現制御機構 (2013)
  • Author(s): 大畑広和、中釜斉、岡本康司
  • Organizer: 第72回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ROCK阻害剤は CD44の発現誘導を介して大腸がん幹細胞の増殖を促進する (2012)
  • Author(s): 大畑広和、石黒竜也、中釜斉、岡本康司
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] ROCK阻害剤はCD44の発現誘導を介して大腸がん幹細胞の増殖を促進する (2012)
  • Author(s): 大畑広和、石黒竜也、中釜斉、岡本康司
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: 札幌