| Project/Area Number |
24790745
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
KINOSHITA Koshi 富山大学, 医学薬学研究部(医学), 助教 (10585920)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 不整脈 / iPS細胞 / QT延長 / 心筋誘導 / 薬剤応答性 / 疾患モデル / 遺伝性心疾患 / LQT / イオンチャネル / パッチクランプ |
|---|
| Research Abstract |
We succeeded in inducing cardiomyocyte from iPS cells using only two chemicals, CHIR99021 and IWP-4. Beating was detected within ten days, similar to the conventional method using Activin A and BMP4 proteins. RT-PCR and immunostaining revealed that the induced cardiomyocyte expressed cardiomyocyte-specific genes or proteins. The alterations of beat rate and field potential duration in response to the adrenergic agonist were comparable between the cardiomyocyte derived from a patient carrying a mutation in KCNQ1 gene and healthy control. Using iPS cell-derived cardiomyocyte, various pharmacological testing will become available in the future.
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