|Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|Outline of Final Research Achievements
We established a stable cell line which expresses low levels of group V sPLA2 with the use of shRNA-knockdown method in Raw264.7 mouse macrophage cell line. This cell line showed delayed- and decreased-activities in uptake and degradation of oxidized-LDL, and these cells also showed decreases of the level of actin polymerization and the activity of Rho GTPase which regulate the uptake of oxidized-LDL into the cells, in comparison with wild type cell line. The expression level and the enzymatic activity of Src were reduced in group V sPLA2-knockdown cell line, and these phenomenons suggested that group V sPLA2 interacts with the promoter region in Src and regulates the expression of Src.