Quantitative Epigenome Mapping to Identify Therapeutic Target in Heart Failure
| Project/Area Number |
24790757
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
HIGO Shuichiro 大阪大学, 医学(系)研究科(研究院), 助教 (00604034)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | エピゲノム / 高速シークエンサー / 心不全 / 次世代シークエンサー |
|---|
| Research Abstract |
To explore a therapeutic target for preventing the progression of heart failure, we established the novel screening method using next generation sequencer. Pressure-overloaded hearts of mice were applied to RNA-sequence and trimethylated histone H3 Lysine 4 (H3K4me3) ChIP-sequence analysis. We developed a quantification algorithm for H3K4me3 marks which enables absolute comparison between control and the pathological conditions. We demonstrate that H3K4me3 accumulated at the genes functionally involved in transcriptional regulation, although the expression levels of these genes were low. We applied the additional H3K4me3 filtering to the transcriptionally upregulated genes in failing hearts and found the genes specifically marked by H3K4me3 under prolonged hypertrophic stimuli. Among them, we identified a transcription factor with significant H3K4me3 enrichment in failing hearts. From these data, now we are currently advancing functional analysis of this molecule.
|
Report
(3 results)
Research Products
(14 results)
-
[Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA22014
Author(s)
Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K
-
Journal Title
Related Report
-
[Journal Article] Noninvasive and quantitative live imaging reveals a potential stress-responsive enhancer in the failing heart2014
Author(s)
Matsuoka K, Asano Y, Higo S, Tsukamoto O, Yan Y, Yamazaki S, Matsuzaki T, Kioka H, Kato H, Uno Y, Asakura M, Asanuma H, Minamino T, Aburatani H, Kitakaze M, Komuro I, Takashima S
-
Journal Title
FASEB J
Volume: 28(4)
Pages: 1870-9
Related Report
-
[Journal Article] Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation2014
Author(s)
Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Takafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y, Takashima S.
-
Journal Title
Proc Natl Acad Sci U S A
Volume: 111(1)
Pages: 7-7
Related Report
-
[Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2.2014
Author(s)
Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K.
-
Journal Title
EMBO Reports
Volume: 15
Issue: 4
Pages: 438-445
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Noninvasive and quantitative live imaging reveals a potential stress-responsive enhancer in the failing heart.2014
Author(s)
Matsuoka K, Asano Y, Higo S, Tsukamoto O, Yan Y, Yamazaki S, Matsuzaki T, Kioka H, Kato H, Uno Y, Asakura M, Asanuma H, Minamino T, Aburatani H, Kitakaze M, Komuro I, Takashima S.
-
Journal Title
FASEB J.
Volume: 28(4)
Issue: 4
Pages: 1870-9
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation2013
Author(s)
Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Tkafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y and Takashima S
-
Journal Title
PNAS
Volume: 111
Issue: 1
Pages: 273-278
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
