The effect of neuromedin U to atherosclerosis caused by chronic inflammation

• Project/Area Number: 24790793
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: HORI Mika 独立行政法人国立循環器病研究センター, 研究所, 上級研究員 (60598043)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 動脈硬化 / コレステロール / マクロファージ / 神経ペプチド / 脂肪性肝炎 / メタボリックシンドローム / ニューロメジンU / 炎症 / 脂質代謝

Outline of Final Research Achievements

Serum total cholesterol level was 2-fold higher in Apoe-/-Nmu-/- mice in Apoe-/- mice by a high-fat diet (HFD) treatment. However, there were no differences between both mice in atherosclerosis progression by both en face and aortic valve section analysis. There were no differences between peritoneal macrophages in both mice in the uptake of ox-LDL or the inflammation induced by ox-LDL treatment.
Nmu-/- mice developed hepatic steatosis induced by HFD, but Apoe-/-Nmu-/- mice did not develop it but increased the number of lipid-laden macrophages. Our findings suggest that NMU has no effect on the development of atherosclerosis, but has significant roles in cholesterol metabolism and some inflammation reaction.

Research Products

• [Journal Article] Association of pancreatic fatty infiltration with pancreatic ductal adenocarcinoma. (2014)
  • Author(s): Hori M, Takahashi M, Hiraoka N, Yamaji T, Mutoh M, Ishigamori R, Furuta K, Okusaka T, Shimada K, Kosuge T, Kanai Y, Nakagama H
  • Journal Title: Clinical and Translational Gastroenterology Volume: 5 Issue: 3 Pages: e53-e53
  • DOI: 10.1038/ctg.2014.5
  • Peer Reviewed / Open Access
• [Journal Article] Invasive ductal carcinoma developing in pancreas with severe fatty infiltration. (2012)
  • Author(s): Hori M, Onaya H, Takahashi M, Hiraoka N, Mutoh M, Kosuge T, Nakagama H
  • Journal Title: Pancreas Volume: 41 Pages: 1137-1139
• [Presentation] Increase of serum VLDL- and LDL-cholesterol and decrease of the number of hepatic macrophages in Apoe-/-Nmu-/- mice (2014)
  • Author(s): Mika Hori, Hitomi Komai, Toshihide Mizuno, Mariko Harada-Shiba
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-26
• [Presentation] Plasma mature and furin-cleaved PCSK9s are removed by LDL apheresis treatment with both LDL adsorption and double membrane filtration (2014)
  • Author(s): Mika Hori, Yumiko Yuasa, Hisashi Makino, Hitomi Komai, Koji Yanagi, Ryo Koezuka, Tamiko Tamanaha, Ichiro Kishimoto, Hiroaki Hattori, Mariko Harada-shiba
  • Organizer: 82nd European atherosclerosis society congress
  • Place of Presentation: スペイン、マドリッド
• [Presentation] Association of neuromedin U with lipid metabolism and atherosclerosis (2013)
  • Author(s): Mika Hori, Toshihide Mizuno, Mariko Harada-Shiba
  • Organizer: 第45回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Protein convertase subtilisin-like/kexin type9(PCSK9) is removed by LDL-apheresis in patients with familial hypercholesterolemia(FH) and arteriosclerosis obliterans(ASO) (2013)
  • Author(s): Yuasa Y, Makino H, Minamino N, Kishimoto I, Tamanaha T, Koezuka R, Hori M, Harada-Shiba M
  • Organizer: 第45回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] ニューロメジンUの脂質代謝及び動脈硬化との関わり (2013)
  • Author(s): 堀 美香、水野 敏秀、斯波 真理子
  • Organizer: 第17回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 大阪
• [Presentation] ニューロメジンUの役割と動脈硬化との関連 (2013)
  • Author(s): 堀美香, 駒井仁美, 水野敏秀, 斯波真理子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Association of neuromedin U with lipid metabolism (2013)
  • Author(s): Mika Hori, Toshihide Mizuno, Mariko Harada-Shiba
  • Organizer: 81st European atherosclerosis society congress
  • Place of Presentation: フランス、リヨン
• [Presentation] Association of neuromedin U with lipid metabolism and atherosclerosis (2013)
  • Author(s): Mika Hori, Toshihide Mizuno, Mariko Harada-Shiba
  • Organizer: 第45回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 日本、東京
• [Presentation] Association of neuromedin U with lipid metabolism (2013)
  • Author(s): Mika Hori, Toshihide Mizuno, Mariko Harada-Shiba
  • Organizer: 81st Congress of the European Atherosclerosis Society
  • Place of Presentation: フランス、リヨン
• [Presentation] The bioactive peptide neuromedin U is associated with lipid metabolism and inflammation (2012)
  • Author(s): 堀 美香、水野 敏秀、斯波 真理子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 日本、福岡