| Project/Area Number |
24790800
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肺胞上皮障害 / 上皮細胞 / びまん性肺胞上皮障害 / 細胞間接着分子 / 急性呼吸不全 |
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| Research Abstract |
Acute respiratory failure is closely related with dysfunction of pulmonary epithelial cells. Especially cell-cell junctions of alveolar epithelial cells are thought to be the barrier of lung, and impairment of cell-cell junctions could fall into respiratory distress. Many cytokines are up-regulated in the acute lung injury; however, how those cytokines affect functions of pulmonary epithelial cell are not clear enough.
Most cell lines of pulmonary epithelial cells derive from cancer cells, and have quite different characters from pulmonary epithelial cells in vivo. We made primary cultures of pulmonary epithelial cells from Rat Lungs. We also revealed that HMGB1 suppressed expression of junction proteins of A549 cells. Now we plan the experiment which will reveal the effect of HMGB1 on primary cells of pulmonary epithelial cells.
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