| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We investigate the roles of CADM1, a member of the immunoglobulin superfamily cell adhesion molecules, in small cell lung cancer (SCLC). Western blotting and RT-PCR analyses have revealed that CADM1 is significantly expressed in 11 of 14 SCLC cells growing in suspension cultures but in neither of 2 SCLC cells showing attached growth to plastic dishes, suggesting that CADM1 is involved in anchorage-independent growth in SCLC. Then, we demonstrate that SCLC expresses a unique splicing variant of CADM1. This variant is almost exclusively observed in SCLC and testis. Interestingly, suppression of CADM1 expression by shRNA reduced spheroid-like cell aggregation of SCLC cells. Immunohistochemistry demonstrates that CADM1 is strongly expressed in 24 of 34 (71%) SCLC. Overexpression of CADM1 was significantly associated with poor prognosis in surgical patients. These findings suggest that CADM1 enhances the malignant features of SCLC, and could provide a molecular target specific to SCLC.
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