Functional analysis of genes associated with cancer stemness in lung cancer cells

• Project/Area Number: 24790808
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: HASE Tetsunari 名古屋大学, 医学部附属病院, 助教 (30621635)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 癌幹細胞 / マイクロRNA / 肺癌

Research Abstract

There are several factors associated with resistance of cancer cells against available therapeutics, one of which seems to be explained by cancer stem cell theory. Previous studies reported cancer stem-like cells were enriched in serum free medium with growth factors and anchorage-independent condition forming cell aggregates called sphere. In this study, we try to identify genes and micro RNAs associated with lung cancer stem cell property by comparing the gene expression of lung cancer cell lines growing in sphere condition with those growing in normal condition.
In lung cancer cell lines growing in sphere culture, the expression of SFTPA2 gene was up-regulated and miR-125b was down-regulated. Ectopic expression of miR-125b did not suppress sphere formation in lung cancer cell line.

Research Products

• [Journal Article] Endobronchial ultrasound transbronchial needle aspiration in older people (2013)
  • Author(s): Okachi S, Imai N, Imaizumi K, Hase T, Shindo Y, Sakamoto K, Aso H, Wakahara K, Hashimoto I, Ito S, Hashimoto N, Sato M, Kondo M, Hasegawa Y
  • Journal Title: Geriatr Gerontol Int Volume: 13(4) Pages: 986-92
  • Peer Reviewed
• [Journal Article] TIMELESS is overexpressed in lung cancer and its expression correlates with poor patient survival (2013)
  • Author(s): Yoshida K, Sato M, Hase T, Elshazley M, Yamashita R, Usami N, Taniguchi T, Yokoi K, Nakamura S, Kondo M, Girard L, Minna JD, Hasegawa Y
  • Journal Title: Cancer Sci Volume: 104(2) Pages: 171-7
  • Peer Reviewed
• [Journal Article] The circadian clock gene BMAL1 is a novel therapeutic target for malignant pleural mesothelioma (2012)
  • Author(s): Elshazley M, Sato M, Hase T, Yamashita R, Yoshida K, Toyokuni S, Ishiguro F, Osada H, Sekido Y, Yokoi K, Usami N, Shames DS, Kondo M, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Int J Cancer15 Volume: 131(12) Pages: 2820-31
  • Peer Reviewed
• [Journal Article] Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells (2012)
  • Author(s): Horio M, Sato M, Takeyama Y, Elshazley M, Yamashita R, Hase T, Yoshida K, Usami N, Yokoi K, Sekido Y, Kondo M, Toyokuni S, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Ann Surg Oncol Volume: 19(Suppl 3:S) Pages: 634-45
  • Peer Reviewed
• [Journal Article] The circadian clock gene BMAL1 is a novel therapeutic target for malignant pleural mesothelioma. (2012)
  • Author(s): Elshazley M, Sato M, Hase T, Yamashita R, Yoshida K, Toyokuni S, Ishiguro F, Osada H, Sekido Y, Yokoi K, Usami N, Shames DS, Kondo M, Gazdar AF, Minna JD, Hasegawa Y
  • Journal Title: Int J Cancer Volume: 131 Pages: 2820-2831
  • Peer Reviewed
• [Presentation] Evaluation of the oncogenic ability of EML4-ALK to transform human bronchial epithelial cells (HBECs) (2013)
  • Author(s): Shigehisa Kajikawa, Mitsuo Sato, Tetsunari Hase, Tomohiko Kakumu, Eiichi Maruyama, Ryo Yamashita, Masashi Kondo, John D. Minna, Yoshinori Hasegawa
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Sydney, Australia
• [Presentation] プロテオミクス解析による悪性胸膜中皮腫バイオマーカー群の同定 (2012)
  • Author(s): 柳澤聖、宇佐美範恭、小野健一郎、近藤征史、長谷哲成、加藤省一、八木香澄、堀田直恵、中村英男、長谷川好規、横井香平、高橋隆
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: さっぽろ芸文館札幌
  • Year and Date: 2012-09-21
• [Presentation] TIMELESS IS OVEREXPRESSED IN LUNG CANCER AND ITS KNOCKDOWN INDUCES APOPTOSIS AND CHEMOSENSITIZATION IN LUNG CANCER CELLS (2012)
  • Author(s): Yoshida K, Sato M, Hase T, Momen Elshazley, Yamashita R, Usami N, Taniguchi T, Yokoi K, Kondo M, Girard L, Minna J, Hasegawa Y
  • Organizer: 5th Asia Pacific Lung Cancer Conference
  • Place of Presentation: Hilton Fukuoka Sea Hawk hotel, Fukuoka