Exploration of less-invasive biomarkers for chronic kidney disease

• Project/Area Number: 24790832
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Tohoku University
• Principal Investigator: SATO Emiko 東北大学, 薬学研究科(研究院), 助教 (20466543)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 慢性腎臓病 / 栄養障害 / 腹膜透析 / 尿毒症物質 / 残存腎機能 / インドキシル硫酸 / メチルグアニジン / グアニジノ化合物 / カルボニル物質 / バイオマーカー

Outline of Final Research Achievements

Residual renal function is an important predictor of 1-year mortality in chronic kidney dialysis patients. In this study, we explored the biomarker of residual renal function (RRF) in peritoneal dialysis patients (PD). Sixteen PD patients (64 ± 11 years, men:10, women;6) were intended to this study. Blood and PD fluids sample were collected in peritoneal equilibration test (PET). Seventeen uremic toxins were quantified by LC-MS/MS. Relationship between plasma uremic toxin concentration and clinical parameters, RRF and InBody data was evaluated. From the results, plasma indoxyl sulfate (IS) and methylguanidine (MG) increased in PD patients in parallel with loss of RRF, but not peritoneal function. Furthermore, plasma concentrations of IS and MG were significantly correlate with body muscle mass. Our data suggested that plasma concentrations of IS and MG were increased with loss of RRF and they affect body muscle.

Research Products

• [Journal Article] A functional (pro)renin receptor is expressed in human lymphocytes and monocytes. (2015)
  • Author(s): Narumi K, Hirose T, Sato E, Mori T, Kisu K, Ishikawa M, Totsune K, Ishii T, Ichihara A, Nguyen G, Sato H, Ito S.
  • Journal Title: Am J Physiol Renal Physiol. Volume: 308 Issue: 5 Pages: F487-F499
  • DOI: 10.1152/ajprenal.00206.2014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of renin-angiotensin system, oxidative stress and inflammation to the blood pressure control in young subjects. (2012)
  • Author(s): Emiko Sato, Takefumi Mori, Yoshimi Nakamichi, Mustuko Fujiwara, Ikuko Oba, Mizuho Tanno, Susumu Ogawa, Yoshitaka Kinouchi, Hiroshi Sato, Wataru Hida, Sadayoshi Ito
  • Journal Title: XVI International Congress on Nutrition and Metabolism in Renal Disease ICRNM Honolulu Volume: June 26-30 Pages: 53-60
• [Presentation] 腹膜透析におけるインドキシル硫酸の動態と意義 (2015)
  • Author(s): 佐藤 恵美子、森 建文、菅原 真恵、大場 郁子、三枝 大輔、小泉 賢治、千田 真貴子、永沼 絵理、佐藤 博、伊藤 貞嘉
  • Organizer: 第58回日本腎臓学会
  • Place of Presentation: 愛知
  • Year and Date: 2015-06-05 – 2015-06-07
• [Presentation] 腹膜透析におけるインドキシル硫酸クリアランスに及ぼす因子の検討 (2014)
  • Author(s): 佐藤 恵美子、森 建文、菅原 真恵、大場 郁子、小泉 賢治、千田 真貴子、永沼 絵理、佐藤 博、伊藤 貞嘉
  • Organizer: 第39回日本医用マススペクトル学会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-16 – 2014-10-17
• [Presentation] 新たな高感度ヘム新たな高感度ヘムオキシゲナーゼ活性測定法オキシゲナーゼ活性測定法 (2014)
  • Author(s): 岩森紗希、佐藤恵美子、吉成浩一、眞野成康、伊藤貞嘉、佐藤博、高橋信行
  • Organizer: 第39回日本医用マススペクトル学会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-16 – 2014-10-17
• [Presentation] 腹膜透析におけるグアニジノ化合物の臨床的研究 (2014)
  • Author(s): 佐藤 恵美子、森 建文、三枝 大輔、菅原 真恵、大場 郁子, 小泉 賢治, 千田 真貴子, 永沼 絵理, 佐藤 博, 伊藤 貞嘉
  • Organizer: 第35回グアニジノ化合物研究会
  • Place of Presentation: 筑波
  • Year and Date: 2014-10-11
  • Invited
• [Presentation] New method for Heme Oxygenase activity assay by LC-MS/MS (2014)
  • Author(s): Iwamori S, Sato E, Yoshinari K, Shimada M, Mano N, Ito S, Sato H, Takahashi N
  • Organizer: The 14th Asian Pacific Congress of Nephrology
  • Place of Presentation: 東京
  • Year and Date: 2014-05-14 – 2014-05-17
• [Presentation] 慢性腎臓病におけるアンジオテンシン代謝物のLC-MS/MSによる一斉分析 (2013)
  • Author(s): 佐藤 恵美子
  • Organizer: 第17回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 大阪
• [Presentation] 若年肥満者の高血圧と尿中バイオマーカーの関連
  • Author(s): 佐藤恵美子、森 建文、米城 淑美、大場 郁子、伊藤 貞嘉
  • Organizer: 第1回臨床高血圧フォーラム
  • Place of Presentation: 大阪
• [Presentation] 若年層の生活習慣病予備群では腎内レニン・アンジオテンシン系、酸化ストレスおよび炎症が亢進しているのか？
  • Author(s): 佐藤恵美子、森 建文、中道淑美、大場郁子、佐藤 博、飛田 渉、伊藤貞嘉
  • Organizer: 第16回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 東京
• [Presentation] ROLE OF RENIN-ANGIOTENSIN SYSTEM AND OXIDATIVE STRESS AND INFLAMMATION TO THE BLOOD PRESSURE CONTROL IN YOUNG SUBJECTS
  • Author(s): Emiko Sato, Takefumi Mori, Yoshimi Yoneki, Ikuko Ooba, Mizuho Tanno, Sadayoshi Ito, Wataru Hida
  • Organizer: 16th International Congress on Renal Nutrition and Metabolism(ICRMM)in Honolulu
  • Place of Presentation: Hawaii, Honolulu, USA