| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Surface protein of mouse podocytes was purified and subjected to immunoblotting. The nitrocellulose membranes were incubated with IgG purified from SLE model mice or its control mice. IgG from the model mice, not the control mice, reacted with 110-kD molecule of podocyte surface proteins, then the band was excised to mass spectrometry analysis. Based on molecules revealed by MASS spectrometry and the molecular size, we identified that integrin beta 1 would be a candidate molecule for an autoantigen on podocytes of SLE model mice. Recombinant mouse integrin beta 1 was then generated. Immunoblotting using this recombinant ITGB1 revealed anti-ITGB1 antibody specifically in the SLE model mice. ELISA was also performed to reveal that the antibody started to elevate at two weeks after spleen cell transfer then peaked at 4 weeks. These data strongly suggest that anti-integrin beta 1 antibodies are generated and deposit on the podocytes in the SLE model mice.
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