| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We established xenotransplantation models that control endogenous mesenchymal stem cell differentiation into mature erythropoietin (EPO)-producing tissue in a niche provided by a developing xenometanephros. Transplantation of rat metanephroi into mouse omentum, and similarly pig metanephroi into cat omentum, led to the recruitment of host cells and EPO production. EPO-expressing cells were not differentiated from integrating vessels because they did not co-express endothelial markers. Instead, EPO-expressing cells were shown to be derived from circulating host cells, as shown by EGFP expression in the grown transplants of chimeric mice bearing bone marrow from a transgenic(tg) mouse expressing EGFP under the control of the EPO promoter.These results suggest that bone marrow cells recruitment and differentiation in a xenotransplanted developing organ. Using metanephroi from tg suicide-inducible mice, the xeno-tissue component could be eliminated, leaving autologous EPO-producing tissue.
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