Creation of an animal model producing anti-aquaporin 4 antibody using NOG mouse

• Project/Area Number: 24790870
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Tohoku University
• Principal Investigator: SUZUKI Chihiro 東北大学, 医学(系)研究科(研究院), 非常勤講師 (20624331)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 自己免疫疾患 / NOGマウス / 自己抗体 / アクアポリン４抗体 / 動物モデル / 視神経脊髄炎

Research Abstract

Our purpose is to create an animal model producing human antibodies, using human cells collected from patients with an autoimmune disease. We injected human peripheral lymph cells or B cells into MHC class I and II Double Knockout NOG mouses. 2month after injection, we weren't able to find the human cells in the blood or the spleen of the mouses. We supposed that human peripheral immune-associated cells need any help from T cells and that too much active T cells do harm to human B cells when those cells live long in the body of mouse. We tested several methods, but we weren't able to find the best way for induction that human B cells can live long.

Research Products

• [Journal Article] Changes in Th17 and regulatory T cells after fingolimod initiation to treat multiple sclerosis (2014)
  • Author(s): 7. Sato DK
  • Journal Title: J Neuroimmunol Volume: 268 Issue: 1-2 Pages: 95-98
  • DOI: 10.1016/j.jneuroim.2014.01.008
  • Peer Reviewed
• [Journal Article] Aquaporin-4 antibody-positive cases beyond current diagnostic criteria for NMO spectrum disorders (2013)
  • Author(s): Sato DK, Nakashima I, Takahashi T, Misu T, Waters P, Kuroda H, Nishiyama S, Suzuki C, Takai Y, Fujihara K, Itoyama Y, Aoki M.
  • Journal Title: Neurology Volume: 80 Issue: 24 Pages: 2210-2216
  • DOI: 10.1212/wnl.0b013e318296ea08
  • Peer Reviewed
• [Journal Article] Neuromyelitis optica should be classified as an astrocytopathic disease rather than a demyelinating disease (2012)
  • Author(s): Fujihara K
  • Journal Title: Clin Exp Neuroimmunol Volume: 3 Issue: 2 Pages: 58-73
  • DOI: 10.1111/j.1759-1961.2012.00030.x
  • Peer Reviewed
• [Presentation] The Importance of a Sensitive AQP4-antibody Assay in Patients Beyond NMO Spectrum Disorders Diagnostic Criteria (2013)
  • Author(s): Douglas Kazutoshi Sato, Ichiro Nakashima, Chihiro Suzuki, Masashi Aoki
  • Organizer: American Academy of Neurology 65th Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2013-03-19
• [Presentation] The Importance of a Sensitive AQP4-antibody Assay in Patients Beyond NMO Spectrum Disorders Diagnostic Criteria
  • Author(s): Douglas Kazutoshi Sato, Ichiro Nakashima, Chihiro Suzuki, and Masashi Aoki
  • Organizer: American Academy of Neurology 65thAnnual Meeting
  • Place of Presentation: San Diego, CA, USA