| Project/Area Number |
24790871
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Tohoku University |
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Principal Investigator |
TAKEUCHI Atsuko 東北大学, 医学(系)研究科(研究院), 助教 (00535239)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | クロイツフェルトヤコブ病 / プリオン / PMCA / プリオン病 / CJD |
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| Research Abstract |
Protein Misfolding Cyclic Amplification (PMCA) is currently one of the most sensitive method of detecting PrPSc, by which protease-resistant isoform of prion protein can be amplified in vitro. However, when human brains were used as substrate, the efficiency of sporadic Creutzfeldt-Jakob CJD (sCJD) prions was considerably lower than that of scrapie-derived hamster-adapted strain. Then, we used human cell lysates overexpressing human PrPC as substrate instead of human brains (cell-PMCA), resulting in the improvement of amplification efficacy of some types of CJD prions. In this study, we amplified CJD prions from a large number of CJD cases including various types with cPMCA. As a result, prions from MV2, VV2 and dura-mata grafted CJD (dCJD) showing the plaque type depositions of PrPSc (p-dCJD) in the brains were strongly amplified with 129V substrate. Interestingly, p-dCJD prions could be amplified efficiently with 129V substrates whereas the genotype of these cases was 129M/M.
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