Anti GM1 antibodies affect the function and the structure of lipid rafts
Project/Area Number |
24790899
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Neurology
|
Research Institution | Fujita Health University |
Principal Investigator |
UEDA AKIHIRO 藤田保健衛生大学, 医学部, 講師 (20600703)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | lipid rafts / GM1ganglioside / ガングリオシド / 中性スフィンゴミエリナーゼ / sphingomyelinase / スフィンゴミエリン / Trk / 抗体 / GM1 / EMRN / Anti neutral glycolipid / neutral glycolipid / ganglioside / Lipid rafts |
Outline of Final Research Achievements |
Important signaling molecules (e.g. Trk-tyrosine kinase etc.) and glycolipids are present inside microdomains named ‘lipid rafts’ in the plasma membrane. Nerve growth factor (NGF) is essential for neuronal differentiation and survival in vivo and its functional receptor is Trk tyrosine kinase. Researchs to date, we have demonstrated that anti-GM1 antibodies directly influence the integrity of lipid rafts. The Trk protein is translocated from the original location of lipid rafts to non-lipid rafts fraction. Previous studies have shown that neutral sphingomyelinase (nSMase) is located in lipid rafts unlike acid sphingomyelinase in lysosomes and is postulated to be an important regulatory factor for the local contents of sphingomyelin in lipid rafts. In the present study, we found that anti-GM1 antibodies caused the alteration of nSMase activity in the plasma membrane fraction. Thus, anti-GM1 antibodies affect not only Trk protein function but also nSMase actions.
|
Report
(4 results)
Research Products
(10 results)
-
[Journal Article] Neurotropin promotes NGF signaling through interaction of GM1 ganglioside with Trk neurotrophin receptor in PC12 cells2015
Author(s)
Fukuda Y, Fukui T, Hikichi C, Ishikawa T, Murate K, Adachi T, Imai H, Fukuhara K, Ueda A, Kaplan AP, Mutoh T
-
Journal Title
Brain Research
Volume: 1596
Pages: 13-21
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Histone deacetylase inhibitor attenuates neurotoxicology of clioquinol in PC12 cells.2015
Author(s)
Fukui T, Asakura K, Hikichi C, Ishikawa T, Murai R, Hirota S, Murate KI, Kizawa M, Ueda A, Ito S, Mutoh T.
-
Journal Title
Toxicology
Volume: 331
Pages: 112-118
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
[Presentation] 抗中性糖脂質抗体陽性の脳脊髄根末梢神経炎(EMRN)の1例2014
Author(s)
植田晃広, 島さゆり, 石川等真, 福井隆男, 村手健一郎, 廣田政古, 引地智加, 木澤真努香, 伊藤信二, 朝倉邦彦, 武藤多津郎
Organizer
第19回日本神経感染症学会学術集会
Place of Presentation
石川県 金沢歌劇座
Year and Date
2014-09-04
Related Report
-
-
-
-