Identification of novel energy metabolism-related genes by MASC assay

• Project/Area Number: 24790917
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Tokyo Medical and Dental University (2014); Tokyo University of Science (2012-2013)
• Principal Investigator: FUJIKAWA Makoto 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (90573048)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
• Keywords: エネルギー代謝制御 / 代謝学 / 生体エネルギー代謝

Outline of Final Research Achievements

It is important for us to regulate accurately biological energy metabolism because of its demand for usual action, thermoregulation and so on. We developed and utilize a screening assay for novel energy metabolic regulators. As a result, it was discovered that the new factor is increased during deprivation of energy and activates the enzyme for energy production. In future, we aim to analyze how the new factor relate to the metabolic disorders which is such as diabetes in order to help prevent and cure those.

Research Products

• [Journal Article] Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ) (2014)
  • Author(s): Fujikawa M, Ohsakaya S, Sugawara K and Yoshida M
  • Journal Title: Genes Cells Volume: 19 Issue: 2 Pages: 153-60
  • DOI: 10.1111/gtc.12121
  • Peer Reviewed
• [Journal Article] Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation (2013)
  • Author(s): Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Tkafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y and Takashima S
  • Journal Title: PNAS Volume: 111 Issue: 1 Pages: 273-278
  • DOI: 10.1073/pnas.1318547111
  • Peer Reviewed / Open Access
• [Journal Article] Screening of protein kinase inhibitors and knockdown experiments identified four kinases that affect mitochondrial ATP synthesis activity (2013)
  • Author(s): Sugawara K, Fujikawa M and Yoshida M
  • Journal Title: FEBS Lett Volume: 587 Issue: 23 Pages: 3843-7
  • DOI: 10.1016/j.febslet.2013.10.012
  • Peer Reviewed
• [Journal Article] Distribution and Dynamics of Intracellular ATP Level, and their Regulation (2013)
  • Author(s): 今村博臣、安東友美、藤川誠
  • Journal Title: Seibutsu Butsuri Volume: 53 Issue: 1 Pages: 020-023
  • DOI: 10.2142/biophys.53.020
  • NAID: 10031139021
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] Assessing the actual contribution of IF1, an inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology and cell viability (2012)
  • Author(s): Fujikawa M, Imamura H, Nakamura J, Yoshida M
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 22 Pages: 18781-18787
  • DOI: 10.1074/jbc.m112.345793
  • Peer Reviewed
• [Journal Article] Effects of thujaplicins on the promoter activities of the human SIRT1 and telomere maintenance factor encoding genes (2012)
  • Author(s): F. Uchiumi, H. Tachibana, H. Abe, A. Yoshimori , T. Kamiya, M. Fujikawa, S. Larsen, A. Honma, S. Ebizuka, S. Tanuma.
  • Journal Title: Pharmaceutica Analytica Acta Volume: 3 Issue: 05 Pages: 159-159
  • DOI: 10.4172/2153-2435.1000159
  • Peer Reviewed
• [Presentation] Novel factors essential for human mitochondrial FoF1-ATP synthase activity found by MASC (Mitochondrial Activity of SLO-permeabilized Cells) screening (2012)
  • Author(s): Fujikawa M and Yoshida M
  • Organizer: EBEC (European Bioenergetics Conference)
  • Place of Presentation: ドイツ (フライブルグ)
• [Presentation] IF1 (mitochondrial FoF1 inhibitor protein); from single molecule to knock-out mouse (2012)
  • Author(s): T. Suzuki, M. Fujikawa, J. Nakamura, M. Yoshida
  • Organizer: EBEC (European Bioenergetics Conference)
  • Place of Presentation: ドイツ (フライブルグ)
• [Presentation] Screening of protein kinase inhibitors that affect ATP synthesis activity using MASC assay (2012)
  • Author(s): K. Sugawara, M. Fujikawa, M. Yoshida
  • Organizer: EBEC (European Bioenergetics Conference)
  • Place of Presentation: ドイツ (フライブルグ)
• [Presentation] Functional identification of unknown genes localized at mitochondria using MASC assay (2012)
  • Author(s): Fujikawa M, Mori M, Sugawara K and Yoshida M
  • Organizer: Japan Society of Cell Biology
  • Place of Presentation: 神戸
• [Presentation] A novel mitochondrial protein m62, which is identified by MASC assay screening, is essential for FoF1-ATP synthase activity and the mRNA level is regulated by the amount of glucose supply (2012)
  • Author(s): Fujikawa M and Yoshida M
  • Organizer: Molecular Biology Society of Japan
  • Place of Presentation: 福岡
• [Book] 波紋疾走 ほとばしる生命エネルギー (2014)
  • Author(s): Makoto Fujikawa
  • Total Pages: 1
  • Publisher: 日本生物工学会