Analysis of nascent islets using a three dimensional imaging method

• Project/Area Number: 24790922
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Kyoto University
• Principal Investigator: FUJIMOTO Hiroyuki 京都大学, 医学(系)研究科(研究院), 助教 (50437274)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: エネルギー・糖質代謝異常 / 膵臓イメージング / 膵島移植 / イメージング / 三次元画像解析 / 新生膵島 / OPT

Research Abstract

The purpose of this study is an establishment of technique to analyze the new islets, pancreatic duct, and blood vessel in pancreas of mouse three-dimensionally using an Optical Projection Tomography (OPT). Islets and blood vessel in pancreas could be constructed three-dimensionally by OPT. And the islets transplantation onto iris of mouse which expressed luciferase protein in islets was occurred to easily observe the nascent islets. The transplanted islets were observed and analyzed by stereomicroscope or IVIS system. As a result, the transplanted islets could be observed and the luciferase activity from islets was evaluated dose dependent manner. Next, agonist for GLP-1 receptor was used for evaluating the nascent islets from transplanted islets. Two weeks injections induce the tendency of increase of luciferase activity derived from transplanted islets which include the nascent islets. Therefore, this method has a possibility to observe and evaluate the nascent islets.

Research Products

• [Journal Article] Substrate-Mediated, High-Efficiency siRNA Electroporation (2014)
  • Author(s): Hiroyuki Fujimoto, Hiroo Iwata
  • Journal Title: Springer Protocols Volume: 1121 Pages: 139-146
  • DOI: 10.1007/978-1-4614-9632-8_12
  • ISBN: 9781461496311, 9781461496328
• [Presentation] 膵β細胞のイメージングを目的としたGLP-1 4 受容体標的111In 標識Exendin-4 誘導体の開発 (2013)
  • Author(s): 神戸香織, 木村寛之, 松田洋和, 小川祐, 豊田健太郎, 藤本裕之, 小野正博, 稲垣暢也, 佐治英郎
  • Organizer: 第23回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 武蔵野大学
  • Year and Date: 2013-06-21
• [Presentation] 膵β細胞のイメージングを目的としたGLP-14受容体標的68Ga標識Exendin(9-39)誘導体の開発 (2013)
  • Author(s): 神戸香織, 木村寛之, 松田洋和, 小川祐, 豊田健太郎, 藤本裕之, 小野正博, 稲垣暢也, 佐治英郎
  • Organizer: 第53回日本核医学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 非侵襲的画像診断法を用いた糖尿病モデル動物の膵β細胞量の解析 (2013)
  • Author(s): 藤本裕之, 豊田健太郎, 木村寛之, 小川祐, 庄暁桐, 平井光春, 松田洋和, 佐治英郎, 稲垣暢也
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本
• [Presentation] 移植後の膵β細胞量モニタリングのための非侵襲的画像診断法の開発 (2013)
  • Author(s): 庄暁桐, 豊田健太郎, 藤本裕之, 木村寛之, 小川祐, 松田洋和, 近藤恭士, 向英里, 天満敬, 平井光春, 岩永康裕, 上本伸二, 佐治英郎, 稲垣暢也
  • Organizer: 第40回膵・膵島移植研究会
  • Place of Presentation: 高松
• [Presentation] 非侵襲的画像診断法を用いた糖尿病モデル動物の膵β細胞量の解析 (2013)
  • Author(s): 藤本裕之、豊田健太郎、木村寛之、小川祐、庄暁桐、平井光春、松田洋和、佐治英郎、稲垣暢也
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本市民会館
• [Presentation] 膵β細胞の分子イメージングを目的とした GLP-1 受容体標的 111In 標識 Exendin-4誘導体の開発 (2013)
  • Author(s): 神戸香織、木村寛之、松田洋和、小川祐、豊田健太郎、藤本裕之、小野正博、稲垣暢也、佐治英郎
  • Organizer: 第23回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 武蔵野大学
• [Presentation] 膵β細胞のイメージングを目的としたGLP-1受容体標的68Ga標識Exendin(9-39)誘導体の開発 (2013)
  • Author(s): 神戸香織, 木村寛之, 松田洋和, 小川祐, 豊田健太郎, 藤本裕之, 小野正博, 稲垣暢也, 佐治英郎
  • Organizer: 第53回日本核医学会学術集会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 移植後の膵β細胞量モニタリングのための非侵襲的画像診断法の開発 (2013)
  • Author(s): 庄暁桐，豊田健太郎，藤本裕之，木村寛之，小川祐，松田洋和，近藤 恭士，向 英里，天満 敬，平井 光春，岩永 康裕，上本 伸二，佐治 英郎，稲垣 暢也
  • Organizer: 第40回 膵・膵島移植研究会
  • Place of Presentation: 高松
• [Presentation] Development of 123I-labeled exendin derivative targeting GLP-1 receptors for insulinoma SPECT imaging (2012)
  • Author(s): Zhuang, X., Toyoda K., Fujimoto H., Kimura H., Ogawa Y., Mukai E., Ueda M., Tenma T., Hirai M., Takagi M., Kon A., Matsuda H., Ono M., Saji H., Innagaki N
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress / 4^t^h Scientific Meeting of the Asian Association for the Study of Diabets
  • Place of Presentation: 京都
• [Presentation] Development of 1Non-Invasive PET Probe for Quantifying Pancreatic β -cell Mass Using Fluorine-18-labeled Exendin-4 (2012)
  • Author(s): Fujimoto H., Toyoda K., Kimura H., Ogawa Y., Mukai E., Zhuang, X., Ueda M., Tenma T., Hirai M., Takagi M., Kon A., Matsuda H., Ono M., Saji H., Innagaki N
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress / 4^t^h Scientific Meeting of the Asian Association for the Study of Diabets
  • Place of Presentation: 京都
• [Presentation] Development of 123I-labeled exendin probe targeting GLP-1 receptors for detecting insulinoma cxells (2012)
  • Author(s): Fujimoto H., Toyoda K., Kimura H., Ogawa Y., Matsuda H., Takagi M., Kon A., Ono M., Saji H., Inagaki N
  • Organizer: 48th EASD Annual Meeting
  • Place of Presentation: ベルリン、ドイツ
• [Presentation] Development of 123I-labeled exendin probe targeting GLP-1 receptors for detecting insulinoma cells (2012)
  • Author(s): Fujimoto, H., Toyoda, K., Kimura, H., Ogawa, Y., Matsuda, H., Takagi, M., Kon, A., Ono, M., Saji, H., Inagaki, N.
  • Organizer: 48th EASD Annual Meeting, Berlin, Germany
  • Place of Presentation: ベルリン
• [Presentation] Development of 123I-Labeled Exendin Derivative Targeting to GLP-1 Receptor for Insulinoma SPECT Imaging (2012)
  • Author(s): Zhuang, X., Toyoda, K., Fujimoto, H., Kimura, H., Ogawa, Y., Mukai, E., Ueda, M., Temma, T., Hirai, M., Takagi, M., Kon, A., Matsuda, H., Ono, M., Saji, H., Inagaki, N.
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress / 4th Scientific Meeting of the Asian Association for the Study of Diabetes
  • Place of Presentation: 京都
• [Presentation] Development of Non-Invasive PET Probe for Quantifying Pancreatic B-Cell Mass Using Fluorine-18-Labeled Exendin-4 (2012)
  • Author(s): Fujimoto, H., Toyoda, K., Kimura, H., Ogawa, Y., Mukai, E., Zhuang, X., Ueda, M., Temma, T., Hirai, M., Matsuda, H., Saji, H., Inagaki, N.
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress / 4th Scientific Meeting of the Asian Association for the Study of Diabetes
  • Place of Presentation: 京都