Analysis of the epigenetic regulation of insulin signaling molecules in pancreatic beta cells.

• Project/Area Number: 24790925
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Kobe University
• Principal Investigator: HASHIMOTO Naoko 神戸大学, 医学(系)研究科(研究院), 研究員 (40559845)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 2型糖尿病 / インスリンシグナル / エピジェネティクス

Research Abstract

We generated a mouse model of low birth weight induced by starvation stress. Specifically, pregnant C57BL/6J mice were subject to a 70% dietary restriction from late pregnancy (10.5 dpc) until delivery. After that, pups of two groups (the mice of food restriction group: RG , and the mice of control group: CG) were fostered to female ICR mice.
The body weight of RG offspring was significantly lower than that of CG offspring at birth. As RG mice exhibited a catch-up growth, the body weight of them became equivalent to that of CG mice about 7 days after birth, and thereafter exceeded that of CG mice. Pancreatic beta cell mass at birth was decreased by approximately 30% in RG offspring as compared to that in CG offspring. A high-fat diet significantly increased the pancreatic beta cell mass in RG mice as compared to that in CG mice at 12 weeks of age. However, RG mice fed on high-fat diets tended to exhibit a decrease in the pancreatic beta cell mass at approximately 20 weeks of age.

Research Products

• [Journal Article] Contribution of insulin signaling to the regulation of pancreatic beta-cell mass during the catch-up growth period in a low birth weight mouse model (2014)
  • Author(s): Yoshida Y, Fuchita M, Kimura-Koyanagi M, Kanno A, Matsuda T, Asahara S, Hashimoto N, Isagawa T, Ogawa W, Aburatani H, Noda T, Seino S, Kasuga M, Kido Y
  • Journal Title: Diabetology International Volume: 5 Pages: 43-52
  • Peer Reviewed
• [Journal Article] Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin (2013)
  • Author(s): Asahara S, Shibutani Y, Teruyama K, Inoue H, Kawada Y, Etoh H, Matsuda T, Kimura-Koyanagi M, Hashimoto N, Sakahara M, Fujimoto W, Takahashi H, Ueda S, Hosooka T, Satoh T, Inoue H, Matsumoto M, Aiba A, Kasuga M, Kido Y
  • Journal Title: Diabetologia Volume: 56 Pages: 1088-1097
  • Peer Reviewed
• [Presentation] Analysis of the epigenetic regulation of insulin signaling molecules in pancreatic beta cells (2012)
  • Author(s): Kawada Y, Asahara S, Sato A, Hashimoto N, Kido Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 膵β細胞量におけるエピジェネティクス制御 (2012)
  • Author(s): 木戸良明
  • Organizer: 第85回日本内分泌学会学術総会シンポジウム
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] インスリンシグナルが低出生体重モデルマウスの膵β細胞量調節に与える影響 (2012)
  • Author(s): 吉田有里
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
• [Presentation] 膵β細胞におけるインスリンシグナルのエピジェネティクス制御の解析 (2012)
  • Author(s): 川田有希奈、淺原俊一郎、佐藤綾香、橋本尚子、木戸良明
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 膵β細胞機能のエピゲノム制御
  • Author(s): 木戸良明
  • Organizer: 第56回日本糖尿病学会学術集会
  • Place of Presentation: 熊本 日航ホテル
  • Invited
• [Presentation] 膵β細胞におけるインスリンシグナルのエピジェネティクス制御の解析
  • Author(s): 川田有希奈、淺原俊一郎、佐藤綾香、橋本尚子、清野進、木戸良明
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本ホテルキャッスル
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fhs-diabetes/