The beneficial role of vaspin in metabolic syndrome by amelioration of ER stress
| Project/Area Number |
24790926
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Okayama University |
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Principal Investigator |
NAKATSUKA Atsuko 岡山大学, 医歯(薬)学総合研究科, 助教 (00625949)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | メタボリックシンドローム / インスリン抵抗性 / 動脈硬化 / 小胞体ストレス / アポトーシス / アディポカイン / 血管内皮細胞 |
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| Research Abstract |
To study the role of vaspin, we generated vaspin transgenic (Tg) mice and vaspin knockout (KO) mice. Under high fat-high sucrose diet, the obesity, insulin resistance and fatty liver were ameliorated in Tg mice, while they were exacerbated in KO mice. We identified GRP78 as a vaspin-interacting molecule, and GRP78 forms complex with anchor proteins on the plasma membrane. On hepatocytes, vaspin interacts with GRP78/MTJ-1 complex and enhance the phosphorylation of Akt and AMPK, and improves glucose and lipid metabolism. Next, we studied the role of vaspin on atherosclerosis. Vaspin inhibited arterial intimal thickening of balloon injured and cuff-injured vessels of rodent model. Vaspin binds to GRP78/VDAC complex on vascular endothelial cells and competes with the known VDAC ligand, kringle 5. Vaspin inhibits kringle 5-induced apoptosis pathway and enhances the phosphorylation of Akt. Subsequently, vaspin inhibits apoptosis of vascular endothelial cells.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] Pemt deficiency ameliorates endoplasmic reticulum stress in diabetic nephropathy2014
Author(s)
Watanabe M, Nakatsuka A, Murakami K, Inoue K, Terami T, Higuchi C, Katayama A, Teshigawara S, Eguchi J, Ogawa D, Watanabe E, Wada J, Makino H
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Journal Title
PLoS ONE
Volume: 9(3)
Issue: 3
Pages: e92647-e92647
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Metallothionein deficiency exacerbates diabetic nephropathy in streptozotocin-induced diabetic mice2014
Author(s)
Tachibana H, Ogawa D, Sogawa N, Asanuma M, Miyazaki I, Terami N, Hatanaka T, Horigushi CS, Nakatsuka A, Eguchi J, Wada J, Yamada H, Takei K, Makino H
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Journal Title
Am J Physiol Renal Physiol
Volume: 306
Issue: 1
Pages: 105-115
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Urinary fetuin-A is a novel marker for diabetic neohropathy in type 2 diabetes identified by lectin microarray2013
Author(s)
Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, Ogawa D, Terami T, Katayama A, Tone A, Iseda I, Hida K, Yamada M, Ogawa T, Makino H
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Journal Title
PLoS ONE
Volume: 8(10)
Issue: 10
Pages: e77118-e77118
DOI
Related Report
Peer Reviewed
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[Journal Article] Vaspin is an adipokine ameliorating ER stress in obesity as a ligand for cell-surface GRP78/MTJ-1 complex2012
Author(s)
Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Horiguchi CS, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
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Journal Title
Diabetes
Volume: 61(11)
Issue: 11
Pages: 2823-2832
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Hydrogen-rich water prevents progression of non-alcoholic steatohepatitis and accompanying hepatocarcinogenesis in mice2012
Author(s)
Kawai D, Takaki A, Nakatsuka A, Wada J, Tamaki N, Yasunaka T, Koike K, Tsuzaki R, Matsumoto K, Miyake Y, Shiraha H, Morita M, Makino H, Yamamoto K
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Journal Title
Hepatology
Volume: 56(3)
Issue: 3
Pages: 912-921
DOI
Related Report
Peer Reviewed
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