AMP-activated protein kinase in macrophage; its potential as a therapeutic target for atherosclerosis
| Project/Area Number |
24790930
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Kumamoto University |
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Principal Investigator |
ISHII Norio 熊本大学, 医学部附属病院, 助教 (10599111)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マクロファージ / AMPK / 動脈硬化 / 細胞周期 |
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| Research Abstract |
In this study, we investigated whether activation of AMP-activated protein kinase (AMPK) could suppress macrophage proliferation, using AICAR, which is a pharmacological AMPK activator. AMPK activation suppressed Ox-LDL-induced macrophage proliferation by suppressing GM-CSF expression and inducing cell cycle arrest. Moreover, treatment with AICAR suppressed atherosclerotic lesion formation, and decreased the number of PCNA-positive macrophages in apolipoprotein E-deficient mice. In conclusion, we found that AICAR-mediated AMPK activation suppressed Ox-LDL-induced macrophage proliferation by suppressing GM-CSF expression and inducing cell cycle arrest, and suppressed the progression of atherosclerosis in apolipoprotein E-deficient mice. Because the proliferation of vascular cells including macrophages is a key event in the development and progression of atherosclerosis, these effects of AMPK activation may represent therapeutic targets for atherosclerosis.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Regulation of TNFα converting enzyme activity in visceral adipose tissue of obese mice.2013
Author(s)
Kawasaki S, Motoshima H, Hanatani S, Takaki Y, Igata M, Tsutsumi A, Matsumura T, Kondo T, Senokuchi T, Ishii N, Kinoshita H, Fukuda K, Kawashima J, Shimoda S, Nishikawa T, Araki E.
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Journal Title
Biochem Biophys Res Commun
Volume: 430
Pages: 1189-1194
Related Report
Peer Reviewed
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[Journal Article] Apocynin suppresses the progression of atherosclerosis in apoE-deficient mice by inactivation of macrophages.2013
Author(s)
Kinoshita H, Matsumura T, Ishii N, Fukuda K, Senokuchi T, Motoshima H, Kondo T, Taketa K, Kawasaki S, Hanatani S, Takeya M, Nishikawa T, Araki E
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Journal Title
Biochem Biophys Res Commun
Volume: 431
Pages: 124-130
Related Report
Peer Reviewed
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