The role of zinc transporter in adipocyte differentiation

• Project/Area Number: 24790936
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Juntendo University
• Principal Investigator: FUKUNAKA Ayako 順天堂大学, 医学部, 研究員 (60586402)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 亜鉛トランスポーター / 脂肪細胞 / 褐色化 / エネルギー消費 / 褐色脂肪 / インスリン感受性 / 肥満

Research Abstract

We investigated the role of ZIP13 in adipocyte tissues, and recently we obtained the following preliminary data.
(1) Zip13-KO mice showed a reduction in subcutaneous WAT mass and visceral WAT mass as expected, while intriguingly the expression levels of several BAT markers including Ucp1 were increased in the subcutaneous WAT from Zip13-KO mice. (2) This browning phenotype of Zip13-KO cells occurs in a cell autonomous manner: namely, preadipocytes derived from subcutaneous WAT of Zip13-KO mice significantly induced the expression of several BAT markers in ex vivo cultures in response to a brown induction cocktail. (3) Zip13-KO mice showed a remarkable obesity-resistant phenotype when fed on a high fat diet. (4) Enhancement of insulin sensitivity was observed in Zip13-KO mice.

Research Products

• [Journal Article] Human IAPP-induced pancreatic beta-cell toxicity and its regulation by autophagy (2014)
  • Author(s): Shigihara N, Fukunaka A, Hara A, Komiya K, Honda A, Uchida T, AbeH, Toyofuku Y, Tamaki M, Ogihara T, Hiddinga HJ, Sakagashira S, Koike M, Uchiyama Y, Yoshimori T, Eberhardt NL, Fujitani Y, Watada H
  • Journal Title: J Clin Invest Volume: (in press)
• [Journal Article] Molecular pathogenesis of Spondylocheirodysplastic Ehlers-Danlos syndrome caused by mutant ZIP13 proteins (2014)
  • Author(s): BinBH, Hojyo S, HosakaT, Bhin J, KanoH, MiyaiT, Ikeda M, Kimura-Someya T, Shirouzu M, Cho EG, Fukue K, Kambe T, Ohashi W, Kim KH, Seo J, Choi DH, Nam YJ, Hwang D, Fukunaka A, Fujitani Y, Yokoyama S, Superti-Furga A, Ikegawa S, Lee TR and Fukada T
  • Journal Title: EMBO Molecular Medicine Volume: (in press)
• [Journal Article] The diabetes susceptible gene SLC30A8/ZnT8 regulates hepatic insulin clearance (2013)
  • Author(s): TamakiM, FujitaniY, HaraA, Uchida T, TamuraY, Takeno K, Kawaguchi M, Watanabe T, Ogihara T, Fukunaka A, Shimizu T, Mita T, Kanazawa A, MO. Imaizumi, Abe T, Kiyonari H, Hojyo S, Fukada T, Kawauchi T, Nagamatsu S, Hiraono T, Kawamori R, Watada H
  • Journal Title: J Clin Invest Volume: 123(10) Pages: 4513-4524
• [Journal Article] Essential role of the zinc transporter Zip9/SLC39A9 in regulating the activation of Akt and Erk in B-cell receptor signaling pathway in DT40 cells (2013)
  • Author(s): TaniguchiM, Fukunaka A, HagiharaM, Watanabe K, Kamino S, Kambe T, Enomoto S, Hiromura M
  • Journal Title: PLoS One Volume: 8(3)
  • NAID: 120005723282
• [Journal Article] The diabetes-susceptible gene SLC30A8/ZnT8 regulates hepatic insulin clearance (2013)
  • Author(s): Tamaki, M., Fujitani, Y, Uchida, T., Tamura, Y., Takeno, K., Kawaguchi, M., Watanabe, T., Hara, A., Ogihara, T., Shimizu, T., Mita, T., Hirose, T., Ohara-Imaizumi, M., Abe, T., Kiyonari, H., Hojyo, S., Fukada, T., Nagamatsu, S., Hirano, T., Kawamori, R., Watada, H.
  • Journal Title: J Clin Invest Volume: 123 Issue: 10 Pages: 4513-4524
  • DOI: 10.1172/jci68807
  • Peer Reviewed
• [Journal Article] Essential role of the zinc transporter ZIP9/SLC39A9 in regulating the activations of Akt and Erk in B-cell receptor signaling pathway in DT40 cells (2013)
  • Author(s): Taniguchi M, Fukunaka A, Hagihara M, Watanabe K, Kamino S, Kambe T, Enomoto S, Hiromura M
  • Journal Title: PLOS ONE Volume: 8 Issue: 3 Pages: e58022-e58022
  • DOI: 10.1371/journal.pone.0058022
  • Peer Reviewed
• [Presentation] ヒトIAPP が誘導する膵β細胞傷害とオートファジーによる制御 (2014)
  • Author(s): 福中彩子, 鴫原奈弓, 原朱美, 小宮幸次, 本田彬, 藤谷与士夫, 綿田裕孝
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
• [Presentation] ヒトIAPP が誘導する膵β細胞傷害とオートファジーによる制御 (2013)
  • Author(s): 福中彩子
  • Organizer: 第3回Metabolism Scientific forum
  • Place of Presentation: 東京
  • Invited
• [Presentation] ヒトIAPP が誘導する膵β細胞傷害とオートファジーによる制御 (2013)
  • Author(s): 福中彩子, 鴫原奈弓, 原朱美, 小宮幸次, 本田彬, 藤谷与士夫, 綿田裕孝
  • Organizer: 第25回分子糖尿病シンポジウム
  • Place of Presentation: 大阪
• [Presentation] Human IAPP とオートファジーの膵β細胞における関連性について (2013)
  • Author(s): 福中彩子
  • Organizer: 第8回Diabetes Research Forum in Tokyo
  • Place of Presentation: 東京
  • Invited
• [Presentation] 亜鉛トランスポーター複合体による亜鉛要求性酵素の活性化機構の解明 (2012)
  • Author(s): 福中彩子
  • Organizer: トランスポーター研究会2012
  • Place of Presentation: 京都
  • Invited
• [Presentation] ヒトIAPPが誘導する膵β細胞傷害とオートファジーによる制御
  • Author(s): 福中彩子
  • Organizer: 第3回 Metabolism Scientific forum
  • Place of Presentation: 東京
  • Invited
• [Presentation] ヒトIAPPが誘導する膵β細胞傷害とオートファジーによる制御
  • Author(s): 福中彩子、鴫原奈弓、原朱美、小宮幸次、本田彬、藤谷与士夫、綿田裕孝
  • Organizer: 第25回分子糖尿病シンポジウム
  • Place of Presentation: 大阪
• [Presentation] ヒトIAPPが誘導する膵β細胞傷害とオートファジーによる制御
  • Author(s): 福中彩子
  • Organizer: 第9回Diabetes research forum in Tokyo
  • Place of Presentation: 東京
  • Invited
• [Book] α細胞の発生・分化と可塑性 内分泌・糖尿病・代謝内科 (2014)
  • Author(s): 福中彩子, 宮塚健, 綿田裕孝
• [Book] 1 型糖尿病 免疫疾患イラストレイティッド 第3部-13 (2013)
  • Author(s): 福中彩子, 藤谷与士夫, 綿田裕孝
  • Publisher: 羊土社
• [Book] 亜鉛と糖尿病 亜鉛の機能と健康 第8章 (2013)
  • Author(s): 福中彩子, 藤谷与士夫
• [Book] VGF に由来するペプチドは膵β細胞の生存と機能を高めることにより2 型糖尿病の発症を抑制するdiabetes update (2013)
  • Author(s): 福中彩子, 綿田裕孝
• [Book] 膵臓の発生におけるRfx6 の役割 内分泌・糖尿病・代謝内科 (2013)
  • Author(s): 福中彩子, 藤谷与士夫, 綿田裕孝
• [Book] 亜鉛の機能と健康 (2013)
  • Author(s): 福中彩子、藤谷与士夫
  • Total Pages: 239
  • Publisher: 建帛社
• [Book] 免疫疾患イラストレイティッド (2013)
  • Author(s): 福中彩子、藤谷与士夫、綿田裕孝
  • Total Pages: 358
  • Publisher: 羊土社
• [Book] Diabetes update (2013)
  • Author(s): 福中彩子、綿田裕孝
  • Total Pages: 71
  • Publisher: メディカルレビュー社
• [Book] 内分泌・糖尿病・代謝内科 (2013)
  • Author(s): 福中彩子、藤谷与士夫、綿田裕孝
  • Total Pages: 6
  • Publisher: 科学評論社
• [Book] 膵β細胞増殖におけるPDGF の役割 内分泌・糖尿病・代謝内科 (2012)
  • Author(s): 福中彩子, 藤谷与士夫, 綿田裕孝
• [Book] 内分泌・糖尿病・代謝内科 (2012)
  • Author(s): 福中彩子、藤谷与士夫、綿田裕孝
  • Total Pages: 4
  • Publisher: 科学評論社