| Project/Area Number |
24790937
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Kitasato University (2013-2014)
独立行政法人国立国際医療研究センター (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | チロシン硫酸化 / 硫酸化チロシン認識抗体 / プロテオミクス解析 / バイオマーカー / 翻訳後修飾 / プロテオーム解析 / 二次元電気泳動 / ウエスタンブロッティング / 質量分析 / 糖尿病性腎症 |
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| Outline of Final Research Achievements |
Although tyrosine sulfation is essential to life, its biological significance is not fully understood. In this study, I first developed the method to stably identify tyrosine sulfated peptides using LC-MS/MS system. Using this method, I analyzed the secreted proteins derived from cultured cells. Large-scale proteomic studies identified several novel proteins which are tyrosine sulfated. Finally, tyrosine sulfated proteins were successfully identified from urine samples. Urine samples have many advantages over traditional blood samples, in terms of easy collection and non-invasive sources. Although further studies are needed to assess the potential clinical importance of the sulfated proteins identified in this study, these findings would promote the detection of disease biomarkers.
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