The study of regulatory mechanisms on ghrelin secretion

• Project/Area Number: 24790941
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Saitama University
• Principal Investigator: SAKATA Ichiro 埼玉大学, 理工学研究科, 准教授 (80610831)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: グレリン / 交感神経 / GPRC5ｂ / グレリン分泌 / 視床下部 / GPR120

Outline of Final Research Achievements

In this study, it has found that these cells express high levels of mRNA encoding G-protein coupled receptor 120 (GPR120). Addition of GW9508 (a GPR120 chemical agonist) and α-linolenic acid (a natural ligand for GPR120) inhibited the secretion of ghrelin. However, the expression levels of preproghrelin and ghrelin O-acyltransferase (GOAT) mRNAs were not influenced by GW9508. Pretreatment with GW-9508 blocked the effect of the norepinephrine (NE)-induced ghrelin elevation in ghrelin cell lines, and GW-9508 inhibited ghrelin secretion via extracellular signal-regulated kinase activity in ghrelin cell lines. These results indicate that the decrease of ghrelin secretion after feeding is induced partially by long-chain fatty acids.

Research Products

• [Journal Article] Neuroanatomical and functional characterization of CRF neurons of the amygdala using a novel transgenic mouse model (2015)
  • Author(s): (2)De Francesco PN, Valdivia S, Cabral A, Reynaldo M, Raingo J, Sakata I, Osborne-Lawrence S, Zigman JM, Perelló M
  • Journal Title: Neuroscience Volume: 289 Pages: 153-165
  • DOI: 10.1016/j.neuroscience.2015.01.006
  • Peer Reviewed
• [Journal Article] Role of calcium and EPAC in norepinephrine-induced ghrelin secretion. (2014)
  • Author(s): Mani BK, Chuang JC, Kjalarsdottir L, Sakata I, Walker AK, Kuperman A, Osborne-Lawrence S, Repa JJ, Zigman JM.
  • Journal Title: Endocrinology Volume: 155 Issue: 1 Pages: 98-107
  • DOI: 10.1210/en.2013-1691
  • Peer Reviewed
• [Journal Article] G-protein coupled receptor 120 (GPR120) signaling regulates ghrelin secretion in vivo and in vitro. (2014)
  • Author(s): Gong Z, Yoshimura M, Aizawa S, Kurotani R, Zigman J, Sakai T, Sakata I.
  • Journal Title: Am J Physiol Endocrinol Metab. Volume: 306 Issue: 1 Pages: 253-260
  • DOI: 10.1152/ajpendo.00306.2013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Seven transmembrane G protein-coupled receptor repertoire of gastric ghrelin cells. (2013)
  • Author(s): Engelstoft MS, Park WM, Sakata I, Kristensen LV, Husted AS, Osborne-Lawrence S, Piper PK, Walker AK, Pedersen MH, Nohr MK, Pan J, Sinz CJ, Carrington PE, Akiyama TE, Jones RM, Tang C, Ahmed K, Offermanns S, Egerod KL, Zigman JM, Schwartz TW.
  • Journal Title: Mol Metab Volume: 4 Issue: 4 Pages: 376-392
  • DOI: 10.1016/j.molmet.2013.08.006
  • Peer Reviewed
• [Journal Article] Angela K. Walker, Won-Mee Park, Jen-Chieh Chuang, Mario Perello, Ichiro Sakata, Sherri Osborne- Lawrence, Jeffrey M. Zigman (2013)
  • Author(s): Characterization of Gastric and Neuronal Histaminergic Populations Using a Transgenic Mouse Model
  • Journal Title: Plos One Volume: 3 Issue: 3 Pages: e60276-e60276
  • DOI: 10.1371/journal.pone.0060276
  • Peer Reviewed
• [Journal Article] Ghrelin increases intracellular Ca2+ concentration in the various hormone-producing cell types of the rat pituitary gland (2012)
  • Author(s): Mami Yamazaki, Sayaka Aizawaa, Toru Tanaka, Takafumi Sakai, Ichiro Sakata.
  • Journal Title: Neuroscience Letters Volume: 526 Issue: 1 Pages: 29-32
  • DOI: 10.1016/j.neulet.2012.07.063
  • Peer Reviewed
• [Journal Article] A major lineage of enteroendocrine cells coexpress CCK, secretin, GIP, GLP-1, PYY, and neurotensin but not somatostatin. (2012)
  • Author(s): Egerod KL, Engelstoft MS, ....... Sakata I, Pedersen J, Windelov JA, Fuchtbauer EM, Olsen J, Sundler F, Christensen JP, Wierup N, Olsen JV, Holst JJ, Zigman JM, Poulsen SS, Schwartz TW.
  • Journal Title: Endocrinology Volume: 153 Issue: 12 Pages: 5782-95
  • DOI: 10.1210/en.2012-1595
  • Peer Reviewed
• [Presentation] G protein-coupled receptor, family C, group 5, member B (GPRC5B) regulation mechanisms on ghrelin signaling (2015)
  • Author(s): Rakhi Chacrabati, Gong Zhi, Daisuke Kondo, Chika Ikenoya, Takafumi Sakai, Ichiro Sakata
  • Organizer: 日本動物学会第6７回関東支部大会
  • Place of Presentation: 早稲田大学・先端生命医科学センター（東京都新宿区）
  • Year and Date: 2015-03-14
• [Presentation] Effect of motilin on gastric acid and pepsin secretion in Suncus murinus (2015)
  • Author(s): Chayon Goswami, Yoshiaki Shimada, Makoto Yoshimura, Anupom Mondal, Sen-ichi Oda, Toru Tanaka, Ichiro Sakata, Takafumi Sakai
  • Organizer: 日本動物学会第6７回関東支部大会
  • Place of Presentation: 早稲田大学・先端生命医科学センター（東京都新宿区）
  • Year and Date: 2015-03-14
• [Presentation] Ghrelin secretion is stimulated via the sympathetic nervous system. (2014)
  • Author(s): Kohei Ueda, Gong Zhi, Takafumi Sakai, Ichiro Sakata
  • Organizer: 第39回日本比較内分泌学会大会・第8回国際両生類爬虫類神経内分泌学会 合同大会
  • Place of Presentation: 岡崎コンファレンスセンター(愛知県岡崎市）
  • Year and Date: 2014-11-08
• [Presentation] Motilin and ghrelin regulate postprandial giant contractions in Suncus murinus (2014)
  • Author(s): K. ITO, A. MONDAL, T. MIKAMI, K. KURODA, T. SAKAI and I. SAKATA
  • Organizer: FNM2014
  • Place of Presentation: 中国 広州
  • Year and Date: 2014-09-05 – 2014-09-07
• [Presentation] Motilin and ghrelin additively stimulate gastric acid secretion in Suncus murinus (2013)
  • Author(s): Ichiro Sakata, Yoshiaki Shimada, Toru Tanaka, Kanako Koike, Sayaka Aizawa, Takafumi Sakai
  • Organizer: Digestive Disease Week (DDW) 2013
  • Place of Presentation: Orlando, Florida,USA
• [Presentation] 迷走神経による消化管収縮運動制御機構―スンクスを用いたin vivo実験― (2013)
  • Author(s): 坂田一郎、宮野祐樹、黒田香百合、小池加奈子、坂井貴文
  • Organizer: 第55回日本平滑筋学会総会
  • Place of Presentation: 旭川市大雪クリスタルホール（北海道）
• [Presentation] Long-chain fatty acids regulate ghrelin secretion via G-protein coupled receptor 120. (2012)
  • Author(s): Zhi Gong, Kazuya Nishina, Sayaka Aizawa, Takafumi Sakai, Ichiro Sakata
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 福井大学
• [Presentation] the regulatory mechanisms of ghrelin secretion and the effect of ghrelin on gastrointestinal motility.
  • Author(s): Ichiro Sakata
  • Organizer: 39th Korean Society for the Study of Obesity (KSSO) annual meeting, Symposium:Gut＆Obesity
  • Place of Presentation: Korea, Seoul
  • Invited
• [Presentation] Regulatory mechanisms of ghrelin secretion.
  • Author(s): Ichiro Sakata:
  • Organizer: 10th Korean PWS Symposium, Korea
  • Place of Presentation: Samsung Medical Center
  • Invited
• [Book] G.I. Research (2013)
  • Author(s): 坂田一郎、坂井貴文
  • Total Pages: 6
  • Publisher: 先端医学社
• [Remarks] 埼玉大学理学部生体制御学科HP
  • URL: http://seitai.saitama-u.ac.jp/
• [Remarks] 埼玉大学理学部生体制御学科細胞制御学研究室HP
  • URL: http://cell.seitai.saitama-u.ac.jp/
• [Remarks] 埼玉大学理学部生体制御学科ホームページ
  • URL: http://seitai.saitama-u.ac.jp/index.html