The study of inflammasome activation system in endocrine and metabolic disorder models.

• Project/Area Number: 24790951
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Jichi Medical University
• Principal Investigator: KAWASHIMA Akira 自治医科大学, 医学部, ポストドクター (60624913)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分解 / ユビキチン / インフラマソーム / ユビキチン化 / 炎症 / 翻訳後修飾 / 内分泌代謝疾患 / プロテオミクス / 分子間相互作用

Outline of Final Research Achievements

The purpose of this study is to identify the inflammasome activation system. Firstly we performed comprehensive analysis of inflammasome components by TOF-MS. In result we found that certain type of E3 ubiquitin ligase binds one of the inflammasome components and ubiquitination. And this molecule inhibits inflammasome activation through degradation of inflammasome components. These results reveal the pathway that regulates the inflammasome through the ubiquitination and degradation.